Abstract: This experiment researched Staphylococcus aureus and pathogenic Escherichia coli of the scale chicken from Handan， Xingtai, Tangshan areas of Hebei,and the sensitivity of disinfectant. According to the characteristics of bacteria cultivation, morphology, biochemistry experiment, animal experiment and glass board agglutinate experiment，7 strains of Staphylococcus aureus and 6 strains of pathogenic E.coli（1 strains O15 , 2 strains O26 , 1 strains O93 and 2 strains O78）were isolated, the air of scale henhouses contains Staphylococcus aureus and pathogenic Escherichia coli, which made the masculine gender rate was 63.6% and 27.27% respectively by statistics. The bacterial sensitivity to the disinfectant was detected by filter method. The sensitivity of two pathogenic bacteria was much different to disinfectant. The results of the test show that the level of air pollution in scale hen houses results from the pathogenic bacteria is different, as well as the sensitivity of disinfectant.

Key words: chicken house; air pollution; Escherichia coli; Staphylococcus aureus; disinfectant