致病性猪链球菌2型的病原分离鉴定及毒力因子的PCR检测

Abstract

Abstract: Grampostive bacterium, identificated with Streptococcus suis serotype 2 by Rapid ID 32 Strep reagent of Streptococcus suis, was isolated in Guangxi. PCR assays were performed for detection of cps2J and three virulence factors, muramidaserelseased protein(MRP), extracelluar factor (EF) and suilysin gene(SLY) of Streptococcus suis. The sequence analysis revealed 98.8% homology to that of cps2J in GenBank. Virulence factors of Streptococcus suis serotype 2 were sly+mrp+ef+, which indicated that the cause of acute death of pigs was highvirulence Streptococcus suis serotype 2 in the pig farm. It was showed that determined bacteria caused infected rats death and leaded to infected rabbits showing temperature hoist to 40.3 ℃ and death due to blood poisoning. 

Key words: Streptococcus suis serotype 2; isolation and identification; virulence factors; PCR

CLC Number:

S852.61+1

HU Jie;;LIU Qi. Isolation and Identification on Pathogens of Swine Streptococcosis and PCR Assays[J]. , 2009, 36(1): 49-52.

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Isolation and Identification on Pathogens of Swine Streptococcosis and PCR Assays

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