›› 2009, Vol. 36 ›› Issue (1): 38-40.

• 生物技术 • Previous Articles     Next Articles

Application of Two Methods for Sitespecific Mutagenesis of Porcine IκBα

LI He-gang1，2，FU Tian1，JIN Er-hui1，CAO Ji-yue2，LI Kui1，XIAO Bang1，YANG Shu-lin1   

  1. 1.Key Laboratory of Domestic Animal Genetic Resources and Germplasm Innovation of CAAS, Institute of Animal Sciences(IAS),Chinese Acadamy of Agricultural Sciences(CAAS),Beijing 100193,China; 2.School of Veterinary Medicine, Huazhong Agricultural University,Wuhan 430070,China
  • Received:1900-01-01 Revised:1900-01-01 Online:2009-01-20 Published:2009-01-20

Abstract: Substitute Ala for the sites of Ser 32 and Ser 36 in porcine IκBα gene,to establish the fundament for mechanism research of the mutant IκBα inhibition on delayed xenograft rejection.QuikChange SiteDirected Mutagenesis Kit was first uesd for the mutagenesis of Ser 32 site,then the kit was subsequently used for the mutagenesis of Ser 36 site,but failed. Finally OEPCR was used for the mutagenesis of Ser 36 site. These two mutagenesis methods were combined to achieve sitespecific mutagenesis at the sites of 〖JP2〗Ser 32 and Ser 36 in porcine IκBα gene.These two methods each has a unique,and each has their own shortcoming.So,in order to get better result by less work,mutation methods should be chosen flexibly according to the real need.

Key words: sitespecific mutagenesis; OEPCR; porcine IκBα

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