鸡传染性法氏囊病病毒VP2蛋白GFP融合分子在COS7细胞内的表达

›› 2008, Vol. 1 ›› Issue (7): 40-42.

• 生物技术 • Previous Articles Next Articles

Expression of VP2 Protein of Infectious Bursal Disease Virus Fused with GFP in COS7 Cells

LI Jiao1,2, ZHANG Hong1,3, ZHANG Gaiping1, WANG Aiping2, YI Minglin1, ZHI Yubao1, LU Kun1, ZHANG Liping1

Henan Key Laboratory for Animal Immunology, Zhengzhou 450002, China; 2.Zhengzhou University, Zhengzhou 450001, China; 3.Henan Educational Institute, Zhengzhou 450003, China

Received:1900-01-01 Revised:1900-01-01 Online:2008-07-20 Published:2008-07-20

Abstract

Abstract: VP2 gene of infectious bursal disease virus(IBDV) was inserted into enhanced GFP expression vector pEGFP-C1. The recombinant eukaryotic expression vector pEGFP-VP2 was constructed, and detected by endonuclease digestion and PCR , and then was ransfected to COS7 cells by lipid reagent. After infecting, green fluorescence was observed under fluorescent microscope. The results indicates that GFP-VP2 fusion protein was successfully expressed in COS7 cells. Stable expressing cells was selected by 200 μg/ml G418, and GFP-VP2 fusion protain was purified by NiHis affinity column.

Key words: chicken; IBDV; VP2 protein; eukaryotic expression; GFP fusion protein

CLC Number:

S852.65+9.4

LI Jiao;;ZHANG Hong;;ZHANG Gaiping;WANG Aiping;YI Minglin;ZHI Yubao;LU Kun;ZHANG Liping. Expression of VP2 Protein of Infectious Bursal Disease Virus Fused with GFP in COS7 Cells[J]. , 2008, 1(7): 40-42.

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Expression of VP2 Protein of Infectious Bursal Disease Virus Fused with GFP in COS7 Cells

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