Abstract: Mitochondrial DNA is the extra nuclear germ plasm in eukaryotic cell. It is characterized as simple construction, the evolutionary rate kept relatively
constant, which has been widely applied to the study of systematic evolution, relative relationship and species identification. To acquire the molecular data of species and derived material identification, the phylogenetic information ,validity and sensitivity of the genes were analyzed. Usually, Francolin mitochondrial COⅡATPase8 gene sequences were amplified by PCR, 1138 bp fragment were cloned and sequenced including cytochrome oxidase subunitⅠpartial sequence, tRNASer,tRNAAsp, cytochrome oxidase subunitⅡ, tRNALeu, ATPase subunit 8 complete sequence and ATPase subunit 6 partial sequence.We found that these sequences were proved to have good homology with other 5 species of poultry recorded in The NCBI. The molecular phylogenetic trees based on the sequence of COⅡ,ATPase8 and tRNA from Francolin and other poultry were constructed by NJ method using sofeware MEGA3.1 showing that the relationships are consistent with the morphological and taxonomic results. And COⅡhad more phylogenetic information than the others’ .