›› 2008, Vol. 1 ›› Issue (12): 56-59.

• 生物技术 • Previous Articles     Next Articles

Cloning of Gene PR-39 from Pig and Construction of PR-39 as Tandem Repeats Expression Vector in E coli

HU Jindong1,WU Yuanzheng1,YANG Hetong1,2,3   

  1. 1.Biotechnology Center of Shandong Academy of Sciences,Key Laboratory for Applied Microbiology of Shandong Province,Jinan 250014,China; 2.Biology Institute of Shandong Academy of Sciences,Jinan 250014,China;3. School of Life Sciences,Shandong University of Technology,Zibo 255049, China
  • Received:1900-01-01 Revised:1900-01-01 Online:2008-12-20 Published:2008-12-20

Abstract: Gene RP39 from pig with the partiality of E coli was synthesized by the method of gene splicing by overlap extension. The fragment was cloned into pMD18T and sequenced. Three copies of gene RP-39 were combinated into express vector pET-Trx. Then pETTrx(PR-39)3 was transformed into E coli BL21 (DE3) and RP39 was inducted which established good foundation for the future development.

Key words: gene synthesis; PR39; tandem repeats; construction of expression vector