Abstract: One virus was isolated from brain and visceral organs of diseased piglets from a pig farm in Bobai of Yulin of Guangxi.The virus that infected rabbits caused typical pseudorabies clinical symptom, induced typical cytopathogenic effect(CPE) after inoculated in PK-15 cell. And virus TCID₅₀ was 10^-7.22/0.1 ml. A specific band with about 947 bp was obtained by PCR amplification witha pair of designed according to a reference.The PCR production was cloned ,sequenced and compared with MinA strain,Ea strain,SH strain,LA strain,GXB strain,GXW strain . The result displayed 98.7% to 99.4% nucleotide sequence identities and 98.1% to 99.1% amino acid sequence identities,so it was comfirmed as pseudorabies virus and it was named GXBB strain. Phylogenetic analysis revealed that the PRV GXBB strain and other PRV strains were closely related and there were few mutations about the epidemic PRV strains in Guangxi at present. The study provides the scientific theoretical basis for prevention and eradication of PRV .

Key words: pseudorabies virus; PK-15 cell; gE gene; isolation and identification; sequence analysis