›› 2008, Vol. 1 ›› Issue (1): 77-79.

• 奶业专栏 • Previous Articles     Next Articles

The Establishment of the Method of the Extraction of RNA from Milk

QIAO Xinan1, ZHU Yancai2, FAN Pei1, DU Junxia1, JIA Haili1, HAN Liqiang1, YANG Guoyu1   

  1. 1Lab of Animal Physiology & Biochemistry, Henan Agricultural University, Zhengzhou 450002,China;2Key Laboratory of Cell Engineering, Henan Institute of Science and Technology, Xinxiang 453003,China
  • Received:1900-01-01 Revised:1900-01-01 Online:2008-01-20 Published:2008-01-20

Abstract: The cow colostrum was applied as the experimental material, and the milk somatic cells were extracted and RNA was extracted and amplicated. The result shows that the colostrum contains a large number of somatic cells(SCC is about 200,000 /ml and the activity of somatic cells is about 60%). The integrality and purity quotient of the extracted RNA can be used to produce DNA. By RT-PCR amplication, the bands of house-keeping gene GAPDH was reasonable, which indicates that produced DNA can be amplificated as the template for other genes. It provides the primary basis of molecular biological analysis for the cow in the aspect of the genetic engineering research.

Key words: cow colostrums; somatic cell from milk; the extraction of RNA

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