›› 2008, Vol. 1 ›› Issue (1): 44-46.

• 生物技术 • Previous Articles     Next Articles

Effects of Neuropeptide Y on Abundance and Activity of Pyruvate Carboxylase mRNA in Vitro Culture Bovine Hepatocyte

CHEN Chengzhen, XIE Guanghong, LIU Guowen, XU Chuang, 
ZHANG Jiabao， WEN Lizheng，WANG Zhe   

  1. College of Animal Science and Veterinary Medicine,Jilin University, Changchun 130062, China
  • Received:1900-01-01 Revised:1900-01-01 Online:2008-01-20 Published:2008-01-20

Abstract: A single factor duplicate test was designed to investigate whether neuropeptide Y affects the expression of pyruvate carboxylase (PC) mRNA and the activity of PC in vitro culture bovine hepatocyte. Neuropeptide Y was added to the media with 0,50,100,200,500,1000 pg/ml. Abuundance of PC mRNA in vitro culture bovine hepatocyte cultured for 12 hours after Neuropeptide Y was added was determined by real-time fluorescence quantitative RT-PCR, and activity of PC was determined by colourimetry. The results showed that the neuropeptide Y could stimulate the expression of PC mRNA was and the activity of PC in vitro culture bovine hepatocyte.

Key words: neuropeptide Y; hepatocyte; pyruvate carboxylase

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