犬瘟热病毒上海株N蛋白编码基因的克隆与序列分析

Abstract

Abstract: According to the reported gene sequence of N protein of Canine Distemper Virus (CDV) in Gene Bank, a special pair of primers were designed to RT
-PCR amplify N protein gene by using the total RNA of CDV SH stain as a template. The results showed that the homology of gene sequence between CDV SH strain and TN strain was 98.9%, A75/17 stain (97.6%), 2544/Han95 strain(96.7%), 98-2654 Strain(96.9%), 5804 strain(96.6%) and Onderstepoort(93.3%) and the homology of coding amino acid between CDV SH strain and the others were respectively 99.0%，98.7%，98.1%，97.9%，97.9% and 96.9%. From this information, it can be predicted
that N protein has highly conservative property.

Key words: canine distemper virus; N protein; cloning; gene sequence

CLC Number:

S852.65+5

ZHANG Yongxia;WANG Jinfu;YU Jinguan;GU Jianxin;SUN Weiping. The Cloning and Sequence Analysis of N Protein Coding Gene of SH Strainof Canine Distemper Virus[J]. , 2007, 34(7): 72-75.

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The Cloning and Sequence Analysis of N Protein Coding Gene of SH Strainof Canine Distemper Virus

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