›› 2007, Vol. 34 ›› Issue (6): 58-60.

• 生理生化 • Previous Articles     Next Articles

The Establishment of ELISA Method for Detecting Porcine Resistin

LENG Yang1,2, LIU You2,LIU Yanfen3,WANG Qun3,PAN Yingbin3,GAO Zhijie3
  

  1. 1.Laboratery Animal Center of Guangdong Medical College,Zhanjiang 524023,China;2.Modern Biochemistry Center of Guangdong Ocean Univercity,Zhanjiang 524088,China;3.Agronomy College of Guangdong Ocean Univercity,Zhanjiang 524088,China
  • Received:1900-01-01 Revised:1900-01-01 Online:2007-06-20 Published:2007-06-20

Abstract: The purified resistin was used as antigen for immunizing rabbit to obtain resistin specific antisera. The ELISA for resistin detection is developed initially by optimizing conditions of ELISA action.The results showed that the working concentration of antigen-antibody was 1∶200 and 1∶400, the lowest detectability of resistin was about 193.75 ng/ml.The established ELISA method was shown to be sensitive,specific and reproducible.

Key words: resistin; recombinant protain; ELISA

CLC Number: