猪伪狂犬病病毒SA株的分离鉴定及其gE基因缺失株转移载体的构建

Abstract

Abstract: The brain Sample of swine that had been doubtfully infected Aujeszky's disease was obtained .The sample was treated and inoculated into Vero and the cell indicated CPE after 7 generations.PCR analysis showed the virus was PRV.The virus was inoculated into other cells and the BHK-21 cell was the optimization for the virus with TCID₅₀ 10^-9.25/0.1 ml .The PM gene fragment that contains YFP express frame,and the PL and PR fragment used for recombination that were cloned from PRV gE gene,were sequentially inserted into pBluescript(M13-) vector.The results of identitication showed that the gE-delection transfer vector had been successfully constructed.

Key words: PRV; isolation and identitication; gE; transfer vector

CLC Number:

S852.65+9.1

GUAN Yu;SHEN Zhiqiang;QU Guanggang;LV Sufang;REN Yanling;LI Shuguang;WANG Yujiong. Isolation and Identitication of PRV SA Strain and Construction of gE-delection Transfer Vector[J]. , 2007, 34(6): 109-111.

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Isolation and Identitication of PRV SA Strain and Construction of gE-delection Transfer Vector

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