›› 2007, Vol. 34 ›› Issue (5): 22-25.

• 动物营养与饲料科学 • Previous Articles     Next Articles

Recent Research Advances of Cloning Transgenic Pigs Rich in Omega-3 Fatty Acids

PAN Dengke1, CHENG Hongxing2, FENG Shutang1, LI Kui1, DENG Jixian2
   

  1. 1.Department of Gene and Cell Engineering, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100094, China; 2. Institute of Biotechnology，Academy of Military Medical Sciences, Beijing 100071, China
  • Received:1900-01-01 Revised:1900-01-01 Online:2007-05-20 Published:2007-05-20

Abstract: In this paper, the significance and rencent progress of transgenic pigs rich in omega-3 fatty acids are presented. In our study，the sFat-1 gene was chemically synthesized according to the sequence from Caenorhabditis briggssae，and constructed into a mammal expression vector pcDNA3.1sFat-lEGFP. Transgenic sFat-1 gene mice were generated by microinjection of vector into pronuclear zygotes. A modified n-3 fatty acid desaturase gene from C. Briggsae produced high proportion of DHA and DPA in transgenic mice. Based on Function Analysis of n-3 Fatty Acid Desaturase Gene sFat-1, porcine fetal fibroblasts were transfected with vector pcDNA3.1sFat-lEGFP. Positive colony was chosed as donors for nuclear transfer to enucleated MⅡoocytes, cloning transgenic pigs rich in omega-3 fatty acids.

Key words: ω-3 fatty acid desaturase gene; polyunsaturated fatty acids; sFat1; transgenic mouse; Cloning pig 

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