Abstract: The homozygotes of 13/17 Robertsonian translocation swine ［2n=36，XY or XX，rob(13;17)］ metaphase spreads were prepared from 3d culture of ConA-stimulated peripheral blood lymphocytes.The single 13/17 Robertsonian translocation chromosome was microdissected,and then amplified by the Linkeradaptor polymerase chain reaction (LA-PCR).After two rounds of PCR amplification ,smear DNA fragments ranging from 200~2800 bp were acquired. Microsatellite analyses (SWR1941 and SWR1120)and Southern Bloting confirmed that the LA-PCR production came from 13/17 Robertsonian translocation chromosome.The research will be useful for intensive molecular linkage map construction of chromosome 13 and 17, and the isolation of important genes located on chromosome 13 and 17.

Key words: swine; robertsonian translocation; microdissection; LA-PCR; microsatellite 