China Animal Husbandry & Veterinary Medicine ›› 2007, Vol. 34 ›› Issue (3): 71-73.

• 遗传繁育 • Previous Articles     Next Articles

Molecular Cloning and Sequence Analyzing of Goose IGF-ⅠGene 5’-UTR

WANG Lei1, WANG Baowei1, JIA Xiaohui2, YANG Zhigang1, LIU Guanglei3, ZHANG Mingai1, ZHANG Xuhui1, LONG Fangyu1
   

  1. 1. Waterfowl Research Institute, Laiyang Agricultural University, Qingdao 266109, China; 2. Animal Husbandry Bureau of Rizhao City in Shandong Province, Rizhao 276826, China; 3. Institute of Animal Science, Chinese Academy of Agricultural Science, Beijing 100094,China
  • Received:1900-01-01 Revised:1900-01-01 Online:2007-03-20 Published:2007-03-20

Abstract: According to the IGF-Ⅰgene 5’-UTR conserved sequences of chicken, duck etc, a pair of degenerate primer was designed to amplify the sequence of Wulong Goose IGF-Ⅰgene 5’-UTR from the genomic DNA. And then the PCR product was subcloned to the pMD18-T vector and sequenced which was 796 bp in length. Compared with the duck and chicken, Wulong Goose IGF-Ⅰgene 5’-UTR sequence shared 98.1％and 93.0％nucleotide homology respectively which indicated that IGF-Ⅰgenes were highly conservative in phylogeny. Moreover, it has 4 bases deletion and 7 bases insertion compared to duck, and 4 bases deletion and 3 bases insertion compared to chicken. There are 5 kind of common restriction sites through nuclear acid DNA sequence by macrorestriction map analyzing.

Key words: goose; IGF-Ⅰgene; 5’-UTR; cloning; sequence analyzing

CLC Number: