China Animal Husbandry and Veterinary Medicine ›› 2023, Vol. 50 ›› Issue (10): 3928-3938.doi: 10.16431/j.cnki.1671-7236.2023.10.006

• Physiological and Biochemical • Previous Articles     Next Articles

Comparative Study on Different Sources of Mesenchymal Stem Cells from White Leghorn Chickens

QIN Xinxin1,2, TENG Wenxi1,2, ZHANG Xiang1,2, YANG Yalin1,2, HU Yuna1,2, WANG Dongyu1,2, RUI Lei3, LI Zandong3   

  1. 1. Jiangxi Provincial Key Laboratory of Environmental Geotechnology and Engineering Disaster Control, Jiangxi Univeirsity of Science and Technology, Ganzhou 341000, China;
    2. Ganzhou Key Laboratory of Basin Pollution Simulation and Control, Jiangxi University of Sicence and Technology, Ganzhou 341000, China;
    3. State Key Laboratory of Agrobiotechnology, College of Biological Science, China Agricultural University, Beijing 100193, China
  • Received:2023-03-14 Online:2023-10-05 Published:2023-09-26

PDF (PC)

106

Abstract: 【Objective】 Avian mesenchymal stem cells (MSCs) were taken as the research target to investigate the differences in biological characteristics and clinical application potential of MSCs from different sources.【Method】 MSCs derived from bone marrow(BM),adipose tissue(AT),umbilical cord (UC) and spleen (S) of four different tissues and organs in White Leghorn chickens were cultured.The expression of cell surface markers was detected by Real-time quantitative PCR,and the population doubling time was observed by cell growth curve.The cytotoxicity of SDF-1 to MSCs from different sources were determined by thiazole blue cytotoxicity assay (MTT),and the in vitro migration of MSCs was detected by Transwell test.The influencing factors of MSCs migration in vitro were determined by Western blotting.Finally,in vitro differentiation of MSCs from different sources were observed by osteogenic differentiation and lipogenic differentiation.【Result】 The morphology of MSCs from different sources were basically consistent.The marker CD29,CD44,CD71,CD90 and CD105 genes were positively expressed.The relative expression of surface markers in MSCs from different sources were different,and the relative expression of CD29 gene was basically the same,the relative expression of CD44 gene in AT-MSCs was significantly higher than that in BM-MSCs (P＜0.05),and the relative expression of CD71 gene in UC-MSCs was significantly lower than that in BM-MSCs (P＜0.05).The relative expression of CD90 gene in UC-MSCs and S-MSCs was significantly lower than that in BM-MSCs (P＜0.01).The relative expression of CD105 gene in S-MSCs was significantly lower than that in BM-MSCs (P＜0.05).The four sources of MSCs all had good growth potential in vitro,with the longest incubation period of 40 h for S-MSCs.All the MSCs from the four sources had strong in vitro migration ability,and the low expression of CXC-chemokine receptor 4(CXCR4) in S-MSCs might be the cause of their low in vitro migration efficiency.MSCs from the four sources all showed in vitro osteogenic and adipogenic differentiation abilities,among which BM-MSCs had strong osteogenic differentiation ability,while S-MSCs had strong adipogenic differentiation ability.【Conclusion】 MSCs could be derived from four sources of tissues and organs in White Lehang chickens,and all had good in vitro proliferation,migration and differentiation ability.The biological characteristics of the four MSCs were different,inducing different clinical application potential.BM-MSCs had superior comprehensive performance,while S-MSCs had significant advantages in adipose induction differentiation.This study strengthened the understanding of avian MSCs,which was of great significance for the study of MSCs treatment of avian related diseases and the protection of rare birds.

Key words: White Leghorn chickens; mesenchymal stem cells; surface markers; cell differentiation; cell migration

CLC Number: