IGFBP-3基因在两个品种鸡组织中的表达及其与生长性状的相关性分析

doi:10.16431/j.cnki.1671-7236.2020.07.023

Abstract

Abstract: To observe the correlation between the expression of the IGFBP-3 gene and growth traits in chickens,the expression profiles of the IGFBP-3 gene of pectoral muscle and liver in two yellow-feathered broiler breeds (Huashan partridge chickens and Qingyuan partridge chickens) differing in growth rate were detected by Real-time quantitative PCR.Results showed that IGFBP-3 mRNA could be detected in pectoral muscle and liver at 9 embryonic day.The expression level of IGFBP-3 mRNA in pectoral muscle in both two chicken breeds was higher than that in liver at the same embryo age.After hatching,the expression level of IGFBP-3 mRNA in liver in two chicken breeds increased rapidly while the expression level of IGFBP-3 mRNA in pectoral muscle decreased.The expression level of IGFBP-3 mRNA in liver in both two chicken breeds was significantly higher than that in pectoral muscle at the same day old (P<0.01).The correlation analysis showed that in both two chicken breeds the mass of body,pectoral muscle and liver were significantly positively correlated with the expression level of IGFBP-3 mRNA in liver (P<0.05;P<0.01),and significantly negatively correlated with the expression level of IGFBP-3 mRNA in pectoral muscle(P<0.01).These results indicated that the expression level of the IGFBP-3 gene in chickens varied in breed,age and organ.The liver produces most IGFBP-3 after hatching,and the expression level of IGFBP-3 mRNA in liver might be important for the body mass and pectoral mass in chickens.

Key words: IGFBP-3 gene; chicken; expression; growth traits

CLC Number:

S831.2

SHAN Yanju, JI Gaige, JU Xiaojun, ZHANG Ming, TU Yunjie, LIU Yifan, ZOU Jianmin, SHU Jingting. Expression of IGFBP-3 Gene in Tissues of Two Chicken Breeds and Its Association with Growth Traits[J]. China Animal Husbandry and Veterinary Medicine, 2020, 47(7): 2181-2189.

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Expression of IGFBP-3 Gene in Tissues of Two Chicken Breeds and Its Association with Growth Traits

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