芒柄花素对免疫抑制小鼠免疫功能影响的研究

doi:10.16431/j.cnki.1671-7236.2020.03.032

Abstract

Abstract: To investigate the effect of formononetin on the immune function in immunosuppressed mice,100 Kunming mice were randomly divided into five groups (20 in per group,half male and half female):normal control group,immunosuppression group,test groupsⅠ,Ⅱ,and Ⅲ,and the medicines were consecutively given to mice by means of intragastric perfusion.The experimental period was 28 d.The mice in immunosuppression group,test groupsⅠ,Ⅱ,and Ⅲ were all given cyclophosphamide (CTX) with 40 μg/(g·BW) for 1-7 d.From 8 to 21 d,the mice in test groupsⅠ,Ⅱ,and Ⅲ were given the solution of formononetin with 50,150 and 250 μg/(g·BW),respectively.While the other mice were given 0.6 mL physiological saline.After the test,thymus index,spleen index,serum hemolysin and the content of IL-2,IL-4 in serum were detected.The surface receptors CD3,CD20 of lymphocyte and the surface receptors CD68 of KCs were measured by immunohistochemistry.The results showed that the immunosuppressed mice were copied successfully with cyclophosphamid,and the formononetin could increase the serum hemolysin,thymus index,spleen index,the content of IL-2 and IL-4 in serum in immunosuppressed mice,and the differences between the immunosuppression group and test group Ⅱ were extremely significant (P<0.01).Immunohistochemical analysis showed that the formononetin could increase CD3 positive T lymphocytes and CD20 positive B lymphocytes significantly in thymus and spleen of immunosuppressed mice,and encourage the proliferation of KCs cells in the liver of immunosuppressed mice,and the differences between the immunosuppression group and test group Ⅱ were also extremely significant (P<0.01).The results suggested that formononetin could improve the immune function of immunosuppressed mice by promoting the proliferation of lymphocytes and releasing the cytokines,and promote obviously the proliferation of KCs in liver.

Key words: formononetin; immunosuppressed mice; immunohistochemical; immune function

CLC Number:

S853.74

ZHANG Yanping, DENG Kang, JIA Ning, FANG Mei, HUANG Weikuan, ZHANG Jianan, LIU Sheng. The Effect of Formononetin on the Immune Function in Immunosuppressed Mice[J]. China Animal Husbandry and Veterinary Medicine, 2020, 47(3): 922-930.

References

[1] 张玉清,王帅,景娇,等.5种中药总黄酮成分体外抗病毒作用观察[J].南京农业大学学报,2012,35(4):105-109. ZHANG Y Q,WANG S,JING J,et al.Anti-virus function of 5 kinds of total flavone ingredients in vitro[J].Journal of Nanjing Agricultural University,2012,35(4):105-109.(in Chinese)
[2] 王尊民,高秀妹,赵庆友,等.梧桐花黄酮的提取及其抑菌、抗病毒效果[J].中国兽医学报,2013,33(2):272-276. WANG Z M,GAO X M,ZHAO Q Y,et al.Antivirus and antibactericidal effects of total flavones extracted from phoenix tree flower[J].Chinese Journal of Veterinary Science,2013,33(2):272-276.(in Chinese)
[3] 周建胜,陈慧,方梅,等.沙冬青种子总黄酮抗病毒活性的研究[J].中国兽医科学,2013,43(4):425-429. ZHOU J S,CHEN H,FANG M,et al.Study on inhibitory effect of the total flavonoids from seeds of Ammopiptanthus on bovine viral diarrhea virus[J].Chinese Veterinary Science,2013,43(4):425-429.(in Chinese)
[4] 钟建青,李波,贾琦,等.天然黄酮类化合物及其衍生物的构效关系研究进展[J].药学学报,2011,46(6):622-630. ZHONG J Q,LI B,JIA Q,et al.Advances in the structure-activity relationship study of natural flavonoids and its derivatives[J].Acta Pharmaceutica Sinica,2011,46(6):622-630.(in Chinese)
[5] 黄伟宽,刘瑞菊,贾宁,等.沙冬青种子总黄酮对免疫抑制小鼠免疫功能及胸腺和脾超微结构的影响[J].畜牧兽医学报,2018,49(8):1752-1760. HUANG W K,LIU R J,JIA N,et al.The effect of total flavonoids from Ammopiptanthus mongolicus on the immune function and ultrastructure of thymus and spleen in immunosuppressed mice[J].Acta Veterinaria et Zootechnica Sinica,2018,49(8):1752-1760.(in Chinese)
[6] 邓康,方梅,贾宁,等.沙冬青种子总黄酮对免疫抑制小鼠的免疫功能及体外脾淋巴细胞增殖的影响[J].中国兽医科学,2018,48(8):1066-1072. DENG K,FANG M,JIA N,et al.Effect of total flavonoids from Ammopiptanthus mongolicus on the immune function in immunosuppressed[J].Chinese Veterinary Science,2018,48(8):1066-1072.(in Chinese)
[7] 刘升,张嘉男,方梅,等.沙冬青种子总黄酮活性成分分析及对小鼠免疫功能的影响[J].中国畜牧兽医,2019,46(9):2726-2736. LIU S,ZHANG J N,FANG M,et al.Analysis on the composition of total flavonoid from the seeds of Ammopiptanthus and the effect on the immune function in mice[J].China Animal Husbandry & Veterinary Medicine,2019,46(9):2726-2736.(in Chinese)
[8] 刘丽娜,李素梅,吴迪,等.芒柄花素抗肿瘤作用机制研究进展[J].黑龙江医药,2017,30(5):953-955. LUI L N,LI S M,WU D,et al.Advances in study on antitumor effect of formononetin[J].Heilongjiang Medicine Journal,2017,30(5):953-955.(in Chinese)
[9] 李自全,孟祥娇.芒柄花素对人非小细胞肺癌细胞增殖、凋亡的影响及相关机制探讨[J].中国实验方剂学杂志,2016,22(20):138-142. LI Z Q,MENG X J.Effect of formononetin on proliferation and apoptosis of human non-small cell lung cancer cell and its relevant mechanism[J].Chinese Journal of Experimental Traditional Medical Formulae,2016,22(20):138-142.(in Chinese)
[10] 万彦军,庄朋伟,张艳军.芒柄花素磺酸钠抗血栓作用及机制研究[J].中国新药杂志,2016,25(12):1355-1358. WAN Y J,ZHUANG P W,ZHANG Y J.Antithrombotic activity of formononetin sodium and its mechanism[J].Chinese Journal of New Drugs, 2016,25(12):1355-1358.(in Chinese)
[11] 周瑞娟,陈红风,叶媚娜,等.芒柄花素对不同亚型乳腺癌细胞增殖及细胞周期的影响[J].肿瘤防治研究,2012,39(9):1051-1055. ZHOU R J,CHEN H F,YE M N,et al.Effects of formononetin on proliferaton and cell cycle of different subtypes of breast cancer cell liness[J].Cancer Research on Prevention and Treatment,2012,39(9):1051-1055.(in Chinese)
[12] 王硕,范祖森.免疫细胞可塑性与免疫病理机制研究进展[J].中国免疫学杂志,2018,34(5):641-645. WANG S,FAN Z S.Research progress on immune cell plasticity and immune pathogenesis[J].Chinese Journal of Immunology,2018,34(5):641-645.(in Chinese)
[13] FURUSAWA Y,OBATA Y,FUKUDA S,et al.Commensal microbe derived butyrate induces the differentiation of colonic regulatory T cells[J].Nature,2013,504(7480):446-450.
[14] 史若男,李婉,宋丽婷,等.动物免疫抑制研究进展[J].中国畜牧兽医,2014,35(9):93-95. SHI R N,LI W,SONG L T,et al.Progress on animal immunosuppression[J].China Animal Husbandry and Veterinary Medicine,2014,35(9):93-95.(in Chinese)
[15] 王洪武,贾亮亮,徐媛青,等.淫羊藿总黄酮对环磷酰胺致免疫低下小鼠的免疫调节作用[J].天津医药,2010,38(12):1068-1071. WANG H W,JIA L L,XU Y Q,et al.Immunoregulatiry,effects of total flavones of epimedium on immunosuppression mice[J].Tianjin Mdeical Journal,2010,38(12):1068-1071.(in Chinese)
[16] 刘继彦,李永强,彭瑞清,等.环磷酞胺单次注射对小鼠淋巴细胞及其亚群的早期作用[J].中国肿瘤生物治疗杂志,2005,12(3):179-182. LIU J Y,LI Y Q,PENG R Q,et al.Changes of lymphocyte and its subsets after a single injection of cyclophosphamide to Balb/c mouse[J].Chinese Journal of Cancer Biotherapy,2005,12(3):179-182.(in Chinese)
[17] 李婉,刘水平,史若男,等.紫锥菊提取物对小鼠免疫器官指数及血清溶血素水平的影响[J].中兽医医药杂志,2015,6:40-42. LI W,LIU S P,SHI R N,et al.Effects of Echinacea purpurea extract on immune organ indexes and serum hemolysin level of mice[J].Journal of Traditional Chinese Veterinary Medicine,2015,6:40-42.(in Chinese)
[18] 萨茹丽,木其尔,王翠芳,等.沙葱黄酮对绵羊外周血淋巴细胞增殖及IL-2、IL-4、IFN-γ mRNA表达的影响[J].畜牧兽医学报,2015,46(6):1063-1070. SA R L,MU Q R,WANG C F,et al.Effect of Allium Mongolicum regel flavonoids on lymphocyte proliferation,mRNA expression of IL-2,IL-4,IFN-γ in peripheral blood lymphocyte of sheep[J].Acta Veterinaria et Zootechnica Sinica,2015,46(6):1063-1070.(in Chinese)
[19] CHOI E J,DEBNATH T,TANG Y,et al.Topical application of Moringa oleifera leaf extract ameliorates experimentally induced atopic dermatitis by the regulation of Th1/Th2/Th17 balance[J].Biomed Pharmacother,2016,84:870-877.
[20] PARK S,KIM J W,YUN H,et al.Mainstream cigarette smoke accelerates the progression of nonalcoholic steato hepatitis by modulating Kupffer cell-mediated hepatocellular apoptosis in adolescent mice[J].Toxicology Letters,2016,256:53-63.
[21] NAITO M,HASEGAWA G,EBE Y,et al.Differentiation and function of Kupffer cells[J].Medical Electron Microscopy,2004,37(1):16-28.
[22] CHEN G S,QI H Z.Effect of Kupffer cells on immune tolerance in liver transplantation[J].Asian Pacific Journal of Tropical Medicine,2012,12:970-972.

The Effect of Formononetin on the Immune Function in Immunosuppressed Mice

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments

[1]	HU Honglian, LI Chao, CHEN Liqing, SONG Liwen, ZHAO Meng, ZHANG Jianxia, WANG Dian, GAO Min. Effects of Dietary β-carotene Supplementation on Lactation Performance, Blood Routine,Serum Biochemical and Immune Indexes of Dairy Cows during the Lactating Peak Period [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(4): 1375-1384.
[2]	LIU Zhen, ZHOU Xueqin, XU Fei, LI Zhefeng, SUN Hanxue, LI Guoqin, TAO Zhengrong, GUAN Mei, ZHENG Qiang, DIAO Xinping, LU Lizhi. Effects of Coated Sodium Butyrate on Growing Performance,Slaughter Performance, Immune Organ Index and Digestive Enzyme Activity of Breeding Pigeons and Squabs [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(3): 998-1006.
[3]	LIU Zhenni, LEI Xiaowen, LI Jianjun, ZENG Qingyuan, TAN Donghai, ZHONG Yunping, ZHANG Qiang, XIE Hualiang. Effects of Fermented Navel Orange Pomace on Intestinal Morphology,Intestinal Mucosal Immune Function and Microbial Diversity of Muscovy Ducks [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(2): 573-585.
[4]	ZHAO Luyuan, ZOU Chenhao, CAO Jiu'ai, GUO Yanshan, LIU Lijun, ZHAN Xiu'an, LIN Gang, LIU Bing, YU Dongyou. Effects of Low Level of Compound Organic Trace Minerals on Serum Biochemistry,Immune Function and Antioxidant Capacity of Meat Ducks [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(1): 86-96.
[5]	LI Hongying, HE Qin, HUANG Enfu, LIANG Qi. Effects of Plant Essential Oils on Immune Function, Antioxidant Capacity and Gut Microbiota of Shan Partridge Ducks [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(7): 2586-2592.
[6]	YUE Fuyuan, ZHU Lianqin, CHEN Fu, ZHANG Beibei, ZHU Fenghua. Effects of Organic Organically Modified Montmorillonite Added to Mildew Feed on Growth Performance and Immune Function in Chicks [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(6): 2137-2144.
[7]	LIU Jiajia, HE Linjiao, JING Hongxin, HAN Tao, MA Lixin, LI Xiaobin. Effects of Supplemental Formononetin on Performance,Blood Gas and Antioxidant Indexes of Yili Horse in 1 000 m Race [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(5): 1738-1745.
[8]	BAI Peitian, KONG Jiamei, PEI Ting, CHENG Zhixue, REN Yuhong. Effects of Compound Microecological Preparation on Growth Performance, Immune Function and Caecal Flora Structure of Weaned Piglets [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(3): 942-952.
[9]	YU Xiaojun, LI Dandan, LI Xuewu, WANG Qun, WANG Guang, WANG Lihua. Effects of Antibacterial Peptide on the Immune Function and Intestinal Tissue Morphology of Growing Female Minks [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(2): 443-453.
[10]	GU Dongdi, DENG Zaishuang, REN Man, SUN Yuhao, GU Youfang, JIN Guangming, JIN Erhui. Effects of Cordyceps Powder Combined with Yeast Selenium on Laying Performance,Egg Quality,Antioxidant and Immune Function in Middle Egg-laying Peak of Laying Hen [J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(7): 2404-2414.
[11]	WANG Yibing, KUANG Zhixiang, YE Jinling, FAN Qiuli, ZHANG Sheng, JIANG Shouqun. Effects of Nano Copper,Spores of Ganoderma lucidum and Soybean Isoflavone on Performance of Growth,Immune and Antioxidation of Qingyuan Partridge Chickens from 1 to 30 Days [J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(7): 2415-2423.
[12]	ZHAO Yi, WEN Luting, HUANG Yin, CHEN Zhong, LIU Qian, LIN Yong, HU Tingjun. Effects of Sophora subprosrate Polysaccharide on Growth Performance and Immune Function of Oreochromis niloticus [J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(10): 3635-3643.
[13]	SONG Yukun, HE Jun. Research Progress on Structure,Function and Its Key Immune Genes of Spleen in Pigs [J]. China Animal Husbandry and Veterinary Medicine, 2020, 47(6): 1709-1718.
[14]	FAN Qiuli, JIANG Shouqun, GOU Zhongyong, LI Long, LIN Xiajing, WANG Yibing, CHEN Fang. Effects of Probiotics,Oligomeric Chitosan,Acidulant and Complex Enzymes on Growth Performance,Immune Function,Carcass Traits and Meat Quality of Yellow-feathered Chicken Aged from 1 to 66 Days Old [J]. China Animal Husbandry and Veterinary Medicine, 2020, 47(5): 1360-1372.
[15]	LUO Yuanqin, LU Yan, NIU Junli, WEI Lianqing, HE Ganggang, ZHENG Xinxia, LU Qicheng, NIU Yujie, ZHANG Wenju. Effects of Cottonseed Peptide-rich Fermented Cottonseed Meal on Growth Performance and Immune Functions in AA Broilers [J]. China Animal Husbandry and Veterinary Medicine, 2020, 47(5): 1395-1403.