滴鼻免疫疫苗对SPF鸡气管功能影响的研究

doi:10.16431/j.cnki.1671-7236.2019.12.033

Abstract

Abstract: In order to study the effect of nasal drip immunization on tracheal function of chickens,tracheal cilia swing rate,tracheal morphology and mucus secretion after nasal drip immunization with Newcastle disease vaccine in SPF chickens were studied.Eighty 7-day-old SPF chickens were randomly divided into control group and test group,40 chickens in each group.The test group received nasal immunization with attenuated Newcastle disease vaccine and the control group received nasal saline.At 0,2,4 and 6 days post immunization,10 chickens were killed randomly in each group.Tracheal cilia swing rate was measured by red blood cell tracer method.Paraffin tissue sections were collected from trachea for HE and Alcian Blue pH 2.5 and pH 1.0 staining.The results showed that the cilia swing rate of SPF chickens in test group was higher than that in control group at 0 and 2 d post immunization,but the difference was not significant (P>0.05).After nasal drip immunization,the trachea structure of SPF chickens was intact without obvious damage.After nasal drip immunization,the distribution volume of acid mucus in trachea of SPF chickens in test group was significantly higher than that of control group at 0 and 2 d post immunization (P<0.05),then decreased significantly,and increased at 6 d post immunization,but the difference was not significant (P>0.05).The distribution volume of sulfated mucus increased significantly at 0 and 2 d post immunization (P<0.05),and then decreased in accordance with the control group.The results showed that the tracheal mucus secretion of SPF chickens increased in two days after nasal drip immunization,and then returned to normal level.There was no significant effect of immunization on tracheal morphology and cilia swing rate.

Key words: nasal drip immunization; SPF chicken; rate of tracheal ciliary oscillation; mucus secretion

CLC Number:

S858.31

WANG Cuicui, ZHOU Shiying, SHI Hao, JIANG Zhiyao, WANG Jianlin. Study on the Effect of Nasal Drip Immunization on the Trachea Function of SPF Chicken[J]. China Animal Husbandry and Veterinary Medicine, 2019, 46(12): 3733-3739.

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Study on the Effect of Nasal Drip Immunization on the Trachea Function of SPF Chicken

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