绵羊Tacr3基因多态性与产羔数的关联分析

doi:10.16431/j.cnki.1671-7236.2019.05.018

Abstract

Abstract:

The aim of this study was to elucidate the association between tachykinin receptor 3 (Tacr3) gene polymorphism (g.21484478A>C,g.21560640C>T and g.21560688T>C loci) and litter size in sheep,providing new genetic markers for high prolific molecular breeding of sheep.Year-round estrous sheep breeds (Small-tail Han sheep,Hu sheep and Cele Black sheep) and seasonal estrous sheep breeds (Tan sheep,Sunite sheep and Prairie Tibetan sheep) were selected and Sequenom MassARRAY^® SNP assay was applied to detect the genotypes of g.21484478A > C,g.21560640C > T and g.21560688T > C loci of Tacr3 gene,then the association was analyzed between Tacr3 gene polymorphism and litter size in Small-tail Han sheep.The results showed that three genotypes were found at g.21484478A>C (AA,CA and CC),g.21560640C>T (TT,TC and CC) and at g.21560688T>C (TT,CT and CC) loci in both year-round estrous and seasonal estrous sheep,both genotype frequency and gene frequency of three loci of Tacr3 gene were extremely significantly different between year-round estrous and seasonal estrous sheep (P<0.01).Population genetic analysis showed that the g.21484478A>C locus was at moderate polymorphism (0.25 < PIC < 0.5) in Tan sheep and Cele Black sheep,it was at low polymorphism (PIC<0.25) in Small-tail Han sheep,Sunite sheep and Hu sheep;The g.21560640C>T locus was at moderate polymorphism in 6 sheep breeds;The g.21560688T>C locus was at low polymorphism in Small-tail Han sheep,Sunite sheep,Hu sheep and Prairie Tibetan sheep.The χ² test indicated that the g.21560640C>T locus of 6 sheep breeds was in Hardy-Weinberg equilibrium (P>0.05);The g.21484478A>C locus of Prairie Tibetan sheep was not in Hardy-Weinberg equilibrium (P<0.05);The g.21560688T>C locus of Tan sheep and Cele Black sheep was not in Hardy-Weinberg equilibrium (P<0.05).The association analysis indicated that the polymorphism of g.21484478A > C,g.21560640C > T and g.21560688T > C had no correlation with the litter size of the first,second or third parity in Small-tail Han sheep (P>0.05).In conclusion,the three polymorphic loci of Tacr3 gene might not be suitable for breeding of Small-tailed Han sheep.

Key words: sheep; Tacr3 gene; genotyping; litter size

CLC Number:

S813.3

CHEN Weihao, TIAN Zhilong, SUN Wei, CHU Mingxing. Polymorphism of Tacr3 Gene and Its Association with Litter Size in Sheep[J]. , 2019, 46(5): 1405-1412.

References

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Polymorphism of Tacr3 Gene and Its Association with Litter Size in Sheep

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