›› 2019, Vol. 46 ›› Issue (5): 1405-1412.doi: 10.16431/j.cnki.1671-7236.2019.05.018

• Genetics and Breeding • Previous Articles     Next Articles

Polymorphism of Tacr3 Gene and Its Association with Litter Size in Sheep

CHEN Weihao1,2, TIAN Zhilong1,3, SUN Wei2, CHU Mingxing1   

  1. 1. Key Laboratory of Animal Genetics, Breeding and Reproduction of Ministry of Agriculture and Rural Affairs, Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, China;
    2. College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China;
    3. College of Animal Science and Technology, Henan University of Science and Technology, Luoyang 471003, China
  • Received:2018-11-01 Online:2019-05-20 Published:2019-05-20

Abstract:

The aim of this study was to elucidate the association between tachykinin receptor 3 (Tacr3) gene polymorphism (g.21484478A>C,g.21560640C>T and g.21560688T>C loci) and litter size in sheep,providing new genetic markers for high prolific molecular breeding of sheep.Year-round estrous sheep breeds (Small-tail Han sheep,Hu sheep and Cele Black sheep) and seasonal estrous sheep breeds (Tan sheep,Sunite sheep and Prairie Tibetan sheep) were selected and Sequenom MassARRAY® SNP assay was applied to detect the genotypes of g.21484478A > C,g.21560640C > T and g.21560688T > C loci of Tacr3 gene,then the association was analyzed between Tacr3 gene polymorphism and litter size in Small-tail Han sheep.The results showed that three genotypes were found at g.21484478A>C (AA,CA and CC),g.21560640C>T (TT,TC and CC) and at g.21560688T>C (TT,CT and CC) loci in both year-round estrous and seasonal estrous sheep,both genotype frequency and gene frequency of three loci of Tacr3 gene were extremely significantly different between year-round estrous and seasonal estrous sheep (P<0.01).Population genetic analysis showed that the g.21484478A>C locus was at moderate polymorphism (0.25 < PIC < 0.5) in Tan sheep and Cele Black sheep,it was at low polymorphism (PIC<0.25) in Small-tail Han sheep,Sunite sheep and Hu sheep;The g.21560640C>T locus was at moderate polymorphism in 6 sheep breeds;The g.21560688T>C locus was at low polymorphism in Small-tail Han sheep,Sunite sheep,Hu sheep and Prairie Tibetan sheep.The χ2 test indicated that the g.21560640C>T locus of 6 sheep breeds was in Hardy-Weinberg equilibrium (P>0.05);The g.21484478A>C locus of Prairie Tibetan sheep was not in Hardy-Weinberg equilibrium (P<0.05);The g.21560688T>C locus of Tan sheep and Cele Black sheep was not in Hardy-Weinberg equilibrium (P<0.05).The association analysis indicated that the polymorphism of g.21484478A > C,g.21560640C > T and g.21560688T > C had no correlation with the litter size of the first,second or third parity in Small-tail Han sheep (P>0.05).In conclusion,the three polymorphic loci of Tacr3 gene might not be suitable for breeding of Small-tailed Han sheep.

Key words: sheep; Tacr3 gene; genotyping; litter size

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