牦牛酪蛋白基因家族的克隆及组织表达分析

doi:10.16431/j.cnki.1671-7236.2019.01.007

Abstract

Abstract:

The aim of this study was to clone the CDS of casein family genes (CSN1S1,CSN1S2,CSN2 and CSN3),and determine their expression in various tissues.Each of 3 healthy,about 4 years old Leiwuqi yak were selected.After slaughter,the tissues of mammary gland,heart,liver and skeletal muscle were collected for the total RNA extraction.The primers for casein family genes were designed and used for gene cloning,and then the sequences were analyzed using bioinformatics.The mRNA expression level of casein family genes were determined using Real-time PCR.The cDNA length of CSN1S1,CSN1S2,CSN2 and CSN3 genes were 919,832,805 and 715 bp,which containing 645,669,690 and 585 bp CDS and encoding 214,222,259 and 194 amino acids,respectively.The yak casein family was closest associated with that of Bos grunniens,followed by Bubalus bubalis,and distant with Sus scrofa.The tissue expression analysis showed that casein family genes were widely expressed in multiply tissues,which had the highest expression level in mammary gland,and followed in skeletal muscle.In addition,there was no difference among CSN1S1,CSN1S2 and CSN2 genes (P > 0.05),but the expression level of CSN2 gene was significantly higher than that of CSN3 gene in mammary gland (P < 0.05).These data provided basic information for the functional research of casein family genes in the regulation of protein metabolism in yak mammary gland,and laid a foundation for the development and utilization of yak genetic resources.

Key words: yak; casein family genes; cloning; tissue expression

CLC Number:

S823.85⁺5

WANG Hui, CHAI Zhixin, ZHONG Jincheng, ZHU Jiangjiang, ZHANG Chengfu. Cloning and Tissue Expression of Casein Family Genes in Yak[J]. , 2019, 46(1): 54-61.

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Cloning and Tissue Expression of Casein Family Genes in Yak

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