成年小鼠Myomaker基因克隆及组织表达分析

doi:10.16431/j.cnki.1671-7236.2018.11.007

Abstract

Abstract:

In order to analyze the expression of Myomaker gene in skeletal muscle of adult mice,the total RNA was extracted from the skeletal muscle of three randomly selected healthy adult mice,and the coding sequence (CDS) of Myomaker gene was amplified by PCR method and analyzed by bioinformatics software.In addition,immunohistochemical and Western blotting methods were used to determine the localization and expression of Myomaker protein in skeletal muscle.The results showed that the CDS region of Myomaker gene was 666 bp,encoding 221 amino acids.The sequence homology of the nucleotide of Myomaker gene in mice were 71.1%,80.7%,83.7%,83.2%,84.1%,86.4%,80.6% and 84.0% comparing with Danio rerio,Gallus gallus,Felis catus,Bos taurus,Canis lupus familiaris,Homo sapiens,Nestor notabilis,Sus scrofa,respectively,and the sequence homology of the amino acids were 75.9%,79.3%,85.5%,85.5%,88.3%,86.9%,81.4% and 93.2%,respectively.The formula of Myomaker protein was C₁₁₅₄H₁₇₆₉N₂₇₅O₂₉₅S₁₈,and the molecular weight,theory isoelectric point,instability index and grand average of hydrophobicity was 24 ku,9.14,39.22 and 0.501,respectively.Immunohistochemical and Western blotting analysis showed that the Myomaker protein expressed in skeletal muscle of healthy adult mice,but it just existed in partial myocyte membrane.This study laid a foundation for the further study of Myomaker gene.

Key words: Myomaker gene; healthy adult mice; skeletal muscle; clone; expression

CLC Number:

Q785

ZHANG Fenghui, LU Meng, LI Yuhong, ZHU Zixin, QIN Jian, LI Yingliang, DU Rong. Cloning and Tissue Expression Analysis of Myomaker Gene in Adult Mice[J]. , 2018, 45(11): 3025-3032.

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Cloning and Tissue Expression Analysis of Myomaker Gene in Adult Mice

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