单增李斯特菌新疆分离株lmo2192基因克隆及生物信息学分析

doi:10.16431/j.cnki.1671-7236.2018.11.005

Abstract

Abstract:

The study was aimed to clone and bioinformatics analysis of lmo2192 gene of Listeria monocytogenes LM90SB2 isolated from diseased sheep in Xinjiang.Specific primers were designed according to the lmo2192 gene sequence in GenBank (accession No.:CAD00270).The PCR method was used to amplify lmo2192 gene.The PCR products were purified and cloned into pMD19-T vector,and then the positive colonies were screened and sequenced,and the nucleotide sequence of lom2192 gene was sequenced and analyzed.The secondary and tertiary structures of the encoded proteins were predicted,and homology and genetic variation analysis were performed.The results showed that LM90SB2 lmo2192 gene was 1 277 bp in length,containing a 969 bp open reading frame (ORF) that encoded 322 amino acids.The nucleotide sequence homology of lmo2192 gene of LM90SB2 strain was 100.0% with CⅡMS-PH-1;99.8% to 99.9% with 81-0861,10-0809,81-0592,81-0558,NTSN,F2365 and WSLC1033;And 97.0% and 96.9% with L2074 and NH1,respectively.The putative amino acid sequence homology was 91.6% to 100.0% with above strains.The phylogenetic tree showed that the lmo2192 gene of LM90SB2 strain was closely related to the serotype 4b strain and clustered into the same branch.The protein secondary structure prediction result showed that LM90SB2 lmo2192 protein was a hydrophilic protein without signal peptide and transmembrane structure.The protein domain predicted result showed that the lmo2192 protein was an ATPase component.The lmo2192 gene of LM90SB2 strain was successfully cloned,it provided theoretical basis for further study of the lmo2192 gene function of LM90SB2.

Key words: Listeria monocytogenes; lmo2192 gene; cloning; bioinformatics analysis

CLC Number:

S852.61

LI Honghuan, QIAN Lingxiao, DU Dongdong, ZHANG Qiwen, LI Qinghui, MA Xun. Cloning and Bioinformatics Analysis of lmo2192 Gene of Listeria monocytogenes from Xinjiang[J]. , 2018, 45(11): 3011-3017.

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Cloning and Bioinformatics Analysis of lmo2192 Gene of Listeria monocytogenes from Xinjiang

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