›› 2018, Vol. 45 ›› Issue (10): 2894-2901.doi: 10.16431/j.cnki.1671-7236.2018.10.027

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Study on Rapid Detection of Chloramphenicol by sol-gel Column in Shrimp

WU Suiqin, HUANG Shan, GAO Cheng, MENG Hui   

  1. School of Environment and Safety Engineering, Jiangsu University, Zhenjiang 212013, China
  • Received:2018-04-24 Online:2018-10-20 Published:2018-10-20

Abstract:

The aim of this study was to establish a sensitive,rapid and specific method for the detection of chloramphenicol (CAP).Based on immunoaffinity chromatography and enzyme-linked immunosorbent assay,a novel immunoaffinity chromatography test column (ICTC) for the rapid detection of CAP in shrimp was developed.The detection layer and control layer of ICTC column were prepared by sol-gel method,and the conditions of detection layer,control layer and CAP-HRP dilution factor were optimized.The performance parameters such as sensitivity and specificity of the ICTC column were determined,and the pretreatment method and matrix interference effect of the shrimp sample were optimized.This study determined that the ICTC column conditions were as follow:The detection layer,anti-CAP sol-gel:blank sol-gel was 10:1 000 (V/V);The control layer,anti-HRP sol-gel:blank sol-gel was 13:1 000 (V/V);CAP-HRP dilution was 1:105.The detection result was determined by the detection layer decolorization method.The detection time was 10 min,and the detection limit of CAP in PBS buffer was 0.5 μg/L.The method had no visible cross-reaction with thiabendazole,florfenicol,florfenicol similar drugs or metabolites.The detection limit of this method in shrimp samples was 0.75 μg/kg.The ICTC column detection method established in this study was fast,sensitive and easy to use,and provided a new technical methods for rapid detection of CAP residues in shrimp on site.

Key words: sol-gel; chloramphenicol; shrimp; immunoaffinity chromatography test column (ICTC)

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