水牛LPIN1基因克隆及序列分析

doi:10.16431/j.cnki.1671-7236.2018.10.001

Abstract

Abstract:

In order to clarify the effect of LPIN1 gene on the fat deposition,reproductive performance and lactation regulation of buffalo,LPIN1 gene was cloned and analyzed by bioinformatics.cDNA was extracted from buffalo ovary,and then LPIN1 gene CDS region was cloned and sequenced.The physical and chemical properties,secondary structure and tertiary structure of lipin1 protein were predicted and analyzed by bioinformatics software.The results showed that the coding region of buffalo LPIN1 gene was 2 793 bp,which encoded 930 amino acids.The buffalo LPIN1 gene nucleotide sequence shared 99.6%,97.9%,97.7%,97.5%,97.4%,97.1%,89.9%,89.8%,86.2% and 83.5% of homology with that of Bubalus bubalis(predicted),Bos mutus,Bos taurus,Capra hircus,Pantholops hodgsonii,Ovis aries,Sus scrofa,Camelus bactrianus,Homo sapiens and Mus musculus,respectively.The buffalo lipin1 protein shared 99%,99%,99%,99%,94%,94% and 90% of similar amino acids sequence with that of Bos taurus,Bos mutus,Capra hircus,Pantholops hodgsonii,Camelus bactrianus,Sus scrofa and Homo sapiens.The results of phylogenetic tree constructed by Mega 5.0 showed that LPIN1 gene in buffalo had a closest relation with Bos taurus,followed by Ovis aries and Capra hircus,indicating LPIN1 gene was highly conserved in different species.The secondary structure of the lipin1 protein was formed with α-helix,β-turn,T-turn and random coil.lipin1 protein was weakly acidic,which mianly located in the nucleus,and had Lipin_N,LNS2 and AF1Q domain and no signal peptide.The Lipin_N and LNS2 were the conservation domain.

Key words: buffalo; LPIN1 gene; cloning; bioinformatics analysis

CLC Number:

Q785

DUAN Anqin, PANG Chunying, DENG Tingxian, LU Xingrong, MA Xiaoya, LIANG Shasha, LIANG Xianwei. Cloning and Sequence Analysis of Buffalo LPIN1 Gene[J]. , 2018, 45(10): 2653-2660.

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Cloning and Sequence Analysis of Buffalo LPIN1 Gene

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