水牛溶血磷脂酸受体3基因的克隆及生物信息学分析

doi:10.16431/j.cnki.1671-7236.2018.05.001

Abstract

Abstract:

The study was aimed to clone and analyze the lysophosphatidic acid receptor 3 (LPAR3) gene of buffalo with bioinformatics method.The ovary was collected from buffalo,DNA samples was extracted and the primers were designed according to the sequence of the buffalo LPAR3 gene (GenBank accession No.:XM_006047539.1).And LPAR3 gene was cloned and analyzed by PCR amplification and bioinformatics softwares.The results showed that LPAR3 gene was 1 552 bp in length containing a CDS sequence of 1 062 bp that encoded 353 amino acids.Sequence homology analysis indicated that the buffalo LPAR3 showed 99.4%,98.9%,98.0%,88.6%,90.0%,90.3% and 91.2% identity with that of Bison bison,Bos taurus,Ovis aries,Sus scrofa,Equus caballus,Rousettus and Cercocebus atys,which was consistent with phylogenetic tree analysis.The formula of the LPAR3 protein was C₁₈₅₃H₂₈₇₈N₄₇₆O₄₈₆S₃₁,and the molecular weight,theory isoelectric point,instability index and grand average of hydrophobicity was 40.59 ku,9.52,47.05 and 0.324,respectively,indicating that it was an alkaline unstable hydrophobic protein.There were 7 transmembrane domain but no signal peptide which showed it was a non-secretory protein.The secondary structure analysis showed that the percentage of α-helices,random coil and extended strand was 48.16%,41.36% and 10.48%,respectively.Protein subcellular localization analysis found that LPAR3 distributed in the plasma membrane (56.5%),endoplasmic reticulum (26.1%),vacuole (8.7%),Golgi apparatus (4.3%) and the nucleus (4.3%), and might play a role in the transport and combination,purines and pyrimidines of transporters and signal transduction.Thus,those results would lay a foundation for clarifying the mechanism of the gene performance in buffaloes.

Key words: buffalo; lysophosphatidic acid receptor 3 (LPAR3); gene cloning; bioinformatics

CLC Number:

PANG Chunying, LIANG Shasha, MA Xiaoya, DENG Tingxian, LU Xingrong, DUAN Anqin, HUANG Yunqi, LIANG Xianwei. Cloning and Bioinformatics Analysis of LPAR3 Gene in Buffalo[J]. , 2018, 45(5): 1127-1136.

References

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Cloning and Bioinformatics Analysis of LPAR3 Gene in Buffalo

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