发酵玉米蛋白粉对奶牛瘤胃体外发酵特性及微生物菌群的影响

doi:10.16431/j.cnki.1671-7236.2018.04.009

Abstract

Abstract:

The objective of this study was to investigate the effect of fermented corn gluten meal (FCGM) on in vitro fermentation characteristic and microorganism population.Three Holstein cows cannulated permanent ruminal fistula with a body weight of (600±25) kg were used to provide rumen fluid.The substrate was total mixed ration (TMR),and FCGM was added to buffered rumen fluid with concentrations of 0,0.3,0.6 and 0.9 g/L,respectively (dry matter basis) with three repetitions per group.Gas production was recorded at 12,24,36 and 48 h of incubation.Moreover,the pH,in vitro dry matter disappearance (IVDMD),cellulase activity,NH₃-N,volatile fatty acids (VFA) and microbial protein concentration of fermentation liquor were determined after 12,24 and 48 h of incubation.After incubation for 24 h,the relative abundance of microorganism was determined.The results showed as followed:①Compared with the control group,adding different levels of FCGM could significantly or extremely significantly improved the gas production (except 12 h),slow gas production,potential gas production and the effective gas production rate (P < 0.05;P < 0.01).②Compared with the control,adding different levels of FCGM could significantly or extremely significantly decreased the pH of the culture medium and increased the content of bacterial protein,VFA and NH₃-N,cellulose enzyme activity and IVDMD (P < 0.05;P < 0.01),and that of 0.9 g/L FCGM group were highest.③The relative expression of the Ruminococcus albus,Ruminococcus flavefaciens,Fibrobacter succinogenes,Streptococcus bovis,Prevotella,Butyrivibrio fibrisolvens,Ruminobacter amylophilus,fungus and protozoa in 0.6 and 0.9 g/L FCGM groups were significant increased (P < 0.05),with that in 0.9 g/L FCGM group were highest.However,the relative abundance of methanogens was significantly decreased compared with the control group (P < 0.05) and that was the lowest in 0.9 g/L FCGM group.In conclusion,the results demonstrated that FCGM could improve gas production,VFA,NH₃-N and microbial protein concentration,increase the relative abundance of cellulolytic bacteria,starch degradation bacteria,proteolytic bacteria and protozoa,improve rumen fermentation,promote the growth of some microbes in the rumen,decrease the relative abundance of methanogens and regulate the population structure of rumen microbe.The optimum dose of FCGM was 0.9 g/L.

Key words: fermented corn gluten meal; in vitro fermentation; fermentation parameter; rumen microbial population

CLC Number:

S816.6

JIANG Xin, ZHANG Liyang, ZHAO Xuejiao, XU Hongjian, ZHANG Yonggen. Effects of Fermented Corn Gluten Meal on in vitro Fermentation Characteristics and Microorganism Population[J]. , 2018, 45(4): 905-915.

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Effects of Fermented Corn Gluten Meal on in vitro Fermentation Characteristics and Microorganism Population

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