2株海洋红酵母的复合诱变及其高产类胡萝卜素菌株的选育

doi:10.16431/j.cnki.1671-7236.2018.02.009

Abstract

Abstract:

In order to screen out mutant strains with high-yield carotenoid, 2 strains of marine Rhodotorula (Rhodotorula mucilaginosa J6 and Rhodotorila glutinis J2) separated and identificated from the coastal area near the Leizhou Peninsula were selected as the starting strains for the combined mutagenesis of nitroso-guanidin (NTG) and ultraviolet (UV) for three times continuously. Initially, the bacterial suspension of the starting strains was treated with NTG for 60 min, and then irradiated with UV for 120 s. After a short time of liquid culture, the suspension of each compound mutation was coated on the agar plate of red yeast, and a certain number of the first, second and third mutant strains were screened out. The mutant strains were inoculated in the red yeast fermentation medium for culturing. After cultured, centrifuged and dried, stem cells were obtained; Mutant strains with high yield carotenoids were separated out in the base of the carotenoid content in stem cells which was determined by adopting spectrophotometry. Eventually, genetic stability test was designed and executed to evaluate the prior combined mutagenesis. The results showed that the forward mutation rate of Rhodotorula mucilaginosa J6 was 56.57% (56/99), there were 3 strains in the third generation, 2 strains in the second generation and only one in the first generation among the 6 high-yield carotenoid mutants. The mutant with highest carotenoid content was Rhodotorula mucilaginosa J6-82 by 99.56% higher than that of the staring strain. Furthermore, in regarding of the Rhodotorula glutinis J2, the forward mutation rate was 53.01% (44/83). In the whole 6 high-yield carotenoid mutants, 4 strains were derived from the third generation and the others were obtained in the second generation. The mutant with the highest carotenoid content was Rhodotorula glutinis J2-75 by 93.64% higher than that of the starting strain. It was worthwhile to conclude that the methods of NTG and UV combined mutagenesis for three times implemented in this study was beneficial to the variation of marine Rhodotorula and significantly increases carotenoid content. Dramatically, the resulted mutant strains had a good genetic stability as mentioned in this paper.

Key words: Rhodotorula mucilaginosa; Rhodotorula glutinis; carotenoid; combined mutagenesis; mutant strain

CLC Number:

TQ926.3

LIU Ying, XIE Weitian, LIU Wenyuan, SONG Lina, XU Chunhou. Combined Mutagenesis of 2 Strains of Marine Rhodotorula and Breeding of the High Carotenoids Producing Strains[J]. , 2018, 45(2): 358-364.

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Combined Mutagenesis of 2 Strains of Marine Rhodotorula and Breeding of the High Carotenoids Producing Strains

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