新疆伊犁地区马疱疹病毒1型ORF30基因序列分析

doi:10.16431/j.cnki.1671-7236.2018.01.008

Abstract

Abstract:

To study the genotypes of EHV-1-XJ2015 strain isolated from Ili area of Xinjiang, this study investigated the potential risk factors for EHV-1-XJ2015.In this experiment, specific primers was designed and using PCR technique to amplify corresponding region of EHV-1-XJ2015 isolate ORF30 gene, connected to vector pMD19-T,and the recombinant plasmid pMD19-T-ORF30 was successfully constructed. Sequencing results showed that EHV-1-XJ2015 strain ORF30 gene 2 254 bp site was A, and encoding asparagine (N).It was proved that EHV-1-XJ2015 was a non-neurotoxic strain, virulent strains tend to behave as a miscarriage. The phylogenetic analysis showed that the EHV-1-XJ2015 strain was the same branch as the 90c16, 00c19, HH1, NY03 isolated from Japan, amino acid homology was the highest and reached 100%. The results provided a theoretical basis for the study of EHV-1 molecular epidemiology and the assessment of existing diagnostic methods and prevention and control measures in Xinjiang, and ultimately achieved the purpose of prevention and control of the disease.

Key words: EHV-1; ORF30; pathogenicity genotype; sequence alignment

CLC Number:

S852.65

FAN Bin, WANG Yan, Zhumahan·A, RAN Duoliang, LIU Jianhua, Jarhen. Sequence Analysis of ORF30 Gene of Equid Herpesvirus Type 1 in Ili of Xinjiang[J]. , 2018, 45(1): 65-70.

References

[1] DAVISON A J, EBERLE R, EHLERS B, et al. The order herpesvirales[J]. Archives of Virology, 2009, 154(1):171.
[2] 杨夷平, 刘建华, 冉多良,等. 马疱疹病毒1型LAMP检测方法的建立[J]. 中国畜牧兽医, 2014, 41(9):35-40. YANG Y P,LIU J H,RAN D L,et al. Establishment of loop-mediated isothermal amplification assay detection method of equine herpesviruses type 1[J]. China Animal Husbandry & Veterinary Medicine,2014, 41(9):35-40.(in Chinese)
[3] WILSON W D. Equine herpesvirus 1 myeloencephalopathy[J]. Veterinary Clinics of North America Equine Practice, 1997, 13(1):53.
[4] CHOWDHURY S I, KUBIN G, LUDWIG H. Equine herpesvirus type 1(EHV-1) induced abortions and paralysis in a Lipizzaner stud:A contribution to the classification of equine herpesviruses[J]. Archives of Virology, 1986, 90(3-4):273.
[5] 阿依吐拉·肉孜, 阿孜古丽, 肖开提,等. 新疆地区马鼻肺炎血清学调查[J]. 中国畜牧兽医文摘, 2013,12:82. AYITULA·ROUZI, AZIGULI,XIAO K T, et al. Serological survey of equine rhinopneumonitis in Xinjiang[J]. Chinese Abstracts of Animal Science and Veterinary Medicine, 2013,12:82.(in Chinese)
[6] 杜润慈, 刘建华, 李静,等. 新疆伊犁部分地区马鼻肺炎血清学调查[J]. 黑龙江畜牧兽医, 2016,22:120-122. DU R C,LIU J H,LI J,et al. Serological survey of equine rhinopneumonitis in parts of Ili prefecture of Xinjiang[J]. Heilongjiang Animal Science and Veterinary Medicine,2016,22:120-122.(in Chinese)
[7] 李娜, 孙建华, 石明,等. 新疆部分地区马鼻肺炎的血清学调查[J]. 动物医学进展, 2014,9:115-117. LI N,SUN J H,SHI M,et al. Serological investigation of equine rhinopneumonitis in part of regions of Xinjiang[J]. Progress in Veterinary Medicine,2014,9:115-117.(in Chinese)
[8] NUGENT J, BIRCHMACHIN I, SMITH K C, et al. Analysis of equid herpesvirus 1 strain variation reveals a point mutation of the DNA polymerase strongly associated with neuropathogenic versus nonneuropathogenic disease outbreaks[J]. Journal of Virology, 2006, 80(8):4047-4060.
[9] GOODMAN L B, LOREGIAN A, PERKINS G A, et al. A point mutation in a herpesvirus polymerase determines neuropathogenicity[J]. PLoS Pathogens, 2007, 3(11):e160.
[10] GR V D W, GOUPIL R C. A single-nucleotide polymorphism in a herpesvirus DNA polymerase is sufficient to cause lethal neurological disease[J]. Journal of Infectious Diseases, 2009, 200(1):20.
[11] KYDD J H, SMITH K C, HANNANT D, et al. Distribution of equid herpesvirus-1(EHV-1) in respiratory tract associated lymphoid tissue:Implications for cellular immunity[J]. Equine Veterinary Journal, 2010, 26(6):470-473.
[12] GOODMAN L B, WAGNER B, FLAMINIO M J, et al. Comparison of the efficacy of inactivated combination and modified-live virus vaccines against challenge infection with neuropathogenic equine herpesvirus type 1(EHV-1)[J]. Vaccine, 2006, 24(17):3636.
[13] STIERSTORFER B, EICHHORN W, SCHMAHL W, et al. Equine herpesvirus type 1(EHV-1) myeloencephalopathy:A case report[J]. Zoonoses & Public Health, 2002, 49(1):37.
[14] 杨永龙, 刘建华, 宋焕堂,等. 新疆伊犁地区马疱疹病毒1型的分离与鉴定[J]. 中国预防兽医学报, 2016, 38(7):550-553. YANG Y L, LIU J H,SONG H T,et al. Isolation and identification of equine herpesvirus 1 in Xinjiang[J].Chinese Journal of Preventive Veterinary Medicine,2016, 38(7):550-553.(in Chinese)
[15] PUSTERLA N, HUSSEY G S. Equine herpesvirus 1 myeloencephalopathy[J]. Veterinary Clinics of North America Equine Practice, 2014, 30(3):489-506.
[16] CAMPBELL E M, HOPE T J. Gene therapy progress and prospects:Viral trafficking during infection[J]. Gene Therapy, 2005, 12(18):1353-1359.
[17] GOEHRING L S, BRANDES K, WITTENBURG L, et al. Anti-inflammatory drugs will decrease infection of endothelial cell with EHV-1[J]. Journal of Equine Veterinary Science, 2012, 32(10):S63.
[18] ABO-SAKAYA R, EBEID M H, GALILA E S M, et al. D/N752 coding change in DNA polymerase gene (ORF30) plays no role in equid herpesvirus type 1(ehv-1) growth in vitro[J]. Benha Veterinary Medical Journal, 2014, 26(2):1-9.
[19] ALLEN G P, BREATHNACH C C. Quantification by Real-time PCR of the magnitude and duration of leucocyte-associated viraemia in horses infected with neuropathogenic vs. non-neuropathogenic strains of EHV-1[J]. Equine Veterinary Journal, 2006, 38(3):252.
[20] VANDEKERCKHOVE A P, GLORIEUX S, GRYSPEERDT A C, et al. Replication kinetics of neurovirulent versus non-neurovirulent equine herpesvirus type 1 strains in equine nasal mucosal explants[J]. Journal of General Virology, 2010, 91(8):2019-2028.
[21] YAMADA S, MATSUMURA T, TSUJIMURA K, et al. Comparison of the growth kinetics of neuropathogenic and nonneuropathogenicequidherpesvirus type 1(EHV-1) strains in cultured murine neuronal cells and the relevance of the D/N (752) coding change in DNA polymerase gene (ORF30)[J]. Journal of Veterinary Medical Science, 2008, 70(5):505-511.

Sequence Analysis of ORF30 Gene of Equid Herpesvirus Type 1 in Ili of Xinjiang

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