产β-甘露聚糖酶内生菌的筛选、鉴定及酶学性质研究

doi:10.16431/j.cnki.1671-7236.2017.08.039

Abstract

Abstract:

An endophyte strain producing β-mannanase was isolated and identified from sprouting konjac seeds,then enzymatic characteristics of β-mannanase was studied. The highest β-mannanase producing strain was obtained by transparent circle preliminary screening and shake flask fermentation secondary screening,and the enzyme activity was evaluated according to dinitrosalicylic acid (DNS) method,and then the strain was identified based on 16S rDNA sequence. The results showed that it was most closely related to Enterobacter ludwigii (99% sequence similarity). The optimal temperature of the β-mannanase was 60℃,and it had a good stability under temperature from 30 to 50℃. The optimal pH of the β-mannanase was 6.0,and it was stable over a range of pH 4.0 to 9.0. The enzyme was slightly activated by Zn²⁺(117.84%),EDTA(115.80%),Cu²⁺(113.76%). On the contrary,Mn²⁺(22.02%) could strongly inhibit the enzyme activity. The Km of the β-mannanase towards konjac flour was 26.65 mg/mL. Furthermore,after 72 h shaking-flask fermentation,the β-mannanase activity achieved 9.48 U/mL,and it had excellent enzymatic characteristics. These superior properties made the β-mannanase a broad application prospects in feed industry.

Key words: β-mannanase; endophyte; enzymatic characterization

CLC Number:

TS201.23

CHEN Zhi-hui, YAN Lin, MO Hai-fei, CHEN Lian-hong, REN Jing-qi, WU Hong. Isolation and Identification of Endophyte Strain Producing β-mannanase and the Studies of Its Characterization[J]. , 2017, 44(8): 2496-2502.

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Isolation and Identification of Endophyte Strain Producing β-mannanase and the Studies of Its Characterization

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