›› 2017, Vol. 44 ›› Issue (8): 2503-2508.doi: 10.16431/j.cnki.1671-7236.2017.08.040

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Making Procedure of Vascular Corrosion Casts of Ovarian Artery in Sheep

YANG Ya-nan, ZHANG Fan, HE Xiao-hui, WANG Xin-rong   

  1. College of Animal Science and Technology, Gansu Agricultural University, Lanzhou 730070, China
  • Received:2017-02-23 Online:2017-08-20 Published:2017-08-18

Abstract:

In order to explore the making procedure of vascular corrosion casts of ovarian artery in sheep and study the distribution of the blood vessels, this study was carried out and it would provide the foundation of blood vessels anatomy of reproductive system including blood supply mechanism of female ovaries in mammals. Firstly, samples of the uterus and appendages in sheep were collected, and then ovarian arteries and their branches were infused with 8% ABS perfusion agents. After the agent became solid, the samples' soft tissues were corroded away with 30% hydrochloric acid, washed them down with running water and the moderate trim process was done for obtaining the complete vascular corrosion casts of ovarian artery in sheep.The finished casts showed that the ovarian artery in sheep arises three branches including uterine, ovarian and tubal. The ovarian branch showed complicated arrangement such as the spiral artery of ovarian hilus, light spiral piece of ovarian hilus and tight spiral piece of ovarian branch. The results confirmed that the ovarian branch was characterized by the presence of a tight spiral configuration in sheep, the ovarian arterioles were fasten on ovarian hilus, and there were some dense capillaries which extend to other regions of the ovary followed them. Through this procedure, the vascular distribution of corrosion casts was clear,three-dimensional and with smooth surface. It could provide a reference or model for the study of ovarian blood vessels in mammals.

Key words: sheep; ovarian artery; infuse

CLC Number: