热带螨重组Blo t 5变应原诱导小鼠过敏模型的建立

doi:10.16431/j.cnki.1671-7236.2017.07.038

Abstract

Abstract:

To development an allergic murine model induced by the recombinant Blo t 5 (rBlo t 5) allergen, the Blo t 5 gene sequences were optimized, synthesized and inserted into the prokaryotic expression vector PQE80L. The Blo t 5 was expressed in E.coli, purified by NI-NTA agarose,and tested by Western blotting. The results showed that the rBlo t 5 could specially react with monoclonal antibodies against Blo t 5. Twelve BALB/c mice were randomly divided into control group (PBS group, n=6) and rBlo t 5-induced group (rBlo t 5 group, n=6). After sensitization by intraperitoneal injection (i.p) and challenged by intratracheal instillation of rBlo t 5, the total serum IgE levels were measured by ELISA; The mice in rBlo t 5 group were challenged with rBlo t 5 by intraperitoneal injection, and the body temperature was measured at regular intervals; The lung was fixed and stained with haematoxylin and eosin (HE).The result showed that compare with PBS group, the total serum IgE levels rised significantly in rBlo t 5 group (P<0.05). The body temperature dropped significantly and a severe allergic reaction (anaphylaxis) was occurred in mice of rBlo t 5 group (P<0.05).The mice in rBlo t 5 group showed inflammatory cell infiltration in airway by HE.These results showed that the allergic murine model was induced successfully by rBlo t 5 allergen.

Key words: Blomia tropicalis; recombinant Blo t 5 (rBlo t 5); allergic model

CLC Number:

R392.8

LI Xiao-ye, XIAO Zheng-pan, LIU Chu, WEI Shuang-shuang, WANG Da-yong, PEI Ye-chun. Development of an Allergic Murine Model Induced by Recombinant Blo t 5 Allergen of Blomia tropicalis[J]. , 2017, 44(7): 2171-2177.

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Development of an Allergic Murine Model Induced by Recombinant Blo t 5 Allergen of Blomia tropicalis

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