N-羟甲基蛋氨酸钙水平对泌乳期奶牛血清含氮物和游离氨基酸的影响

doi:10.16431/j.cnki.1671-7236.2017.03.014

Abstract

Abstract:

To study the effects of N-hydroxymethyl methionine calcium (N-HMM-Ca) supplementation levels on serum nitrogenous compounds and free amino acids of lactating dairy cows. 30 lactating Holstein dairy cows with similar parities,milk yield and days in milk were randomly assigned into 5 groups. The control group was fed with basal diet,test groups were fed with basal diet supplemented with 0.15%,0.30%,0.75% and 1.50% N-HMM-Ca,respectively. The experiment was lasted for 56 d,including 14 d of adaptation period and 42 d of experimental period. The results showed as follows:①Comparing with control group,the total protein,albumin and globulin in experimental groups were not significantly affected (P>0.05).On the 14th day,serum creatinine concentration in 1.5% level group was significantly lower than control group (P<0.05);On the 42th day,urea nitrogen concentration in 1.5% level group was significantly lower than control group (P<0.05),while the concentration of uric acid in 0.75% level group was extremely significantly higher than that in other groups (P<0.01).②On the 14th day,the concentration of serum Glu,Ser,Arg,Leu,Ile,Lys and total free amino acids in 0.15% level group was highest,which were significantly or extremely significantly higher than control group (P<0.05;P<0.01).On the 42th day,serum Arg,Cys and the total free amino acids in 0.15% level group were the highest. And serum Glu,Ser,Tyr,Met,Val and Lys in 0.30% level group were highest,which were significantly or extremely significantly higher than the control group (P<0.05;P<0.01). In summary, adding 0.15% or 0.30% N-HMM-Ca on diet of lactating dairy had no significant effect on serum nitrogen content (P>0.05),but adding 0.15% N-HMM-Ca could increase the concentration of serum free amino acids. Considering all the facts,the recommended amount of N-HMM-Ca on diet of lactating dairy cows was 0.15%.

Key words: lactating dairy cows; N-HMM-Ca; nitrogenous compounds; free amino acids

CLC Number:

S816.71

FANG Wei, MA Ting-ting, CHEN Zhi-yuan, ZHAN Jin-shun, LI Hong-gen, HUO Yong-jiu, ZHAO Guo-qi. Effects of N-hydroxymethyl Methionine Calcium Supplementation Levels on Serum Nitrogenous Compounds and Free Amino Acids of Lactating Dairy Cows[J]. , 2017, 44(3): 717-723.

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Effects of N-hydroxymethyl Methionine Calcium Supplementation Levels on Serum Nitrogenous Compounds and Free Amino Acids of Lactating Dairy Cows

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