铜绿假单胞菌生物膜研究进展

doi:10.16431/j.cnki.1671-7236.2016.11.025

Abstract

Abstract:

The opportunistic pathogen Pseudomonas aeruginosa is a gram-negative bacteria which can form biofilms.In this review,we summarized the biology mechanism of biofilms in Pseudomonas aeruginosa,including Pseudomonas aeruginosa adhesion,extracellular polysaccharide Psl and Pel,alginate participated in the biofilm maturation,and quorum sensing systems regulated the formation of biofilm in Pseudomonas aeruginosa,and the drug target on biofilm.The bacterial biofilms protected the bacteria from unsuitable environmental conditions.Through researching the structure and the pathogenesis of Pseudomonas aeruginosa,and its resistance molecular mechanisms,we could adjust or regulate the expression of biofilm-associated factors,optimize the anti-infection treatment in Pseudomonas aeruginosa.

Key words: Pseudomonas aeruginosa; biofilms; quoring sensing system

CLC Number:

R378.99⁺1

MENG Fei, LI Shu-chang, YANG Wu-ning, LIU Wei, YIN Hui-hui, WEI Xiao-jie, JIANG Yuan-ming, ZHAO Wu, QIN Zhen-hua, SUN Jian-hua. Research Progress on Biofilms in Pseudomonas aeruginosa[J]. , 2016, 43(11): 2976-2981.

References

[1] Lawrence R M,Vincent M I,Kim L.Pseudomonas aeruginosa biofilms in disease[J].Microb Ecol,2014,68(1):1-12.
[2] Bohn Y S,Brandes G,Rakhimova E,et al.Multiple roles of Pseudomonas aeruginosa TBCF10839 PilY1 in motility,transport and infection[J].Mol Microbiol,2009,71(3):730-747.
[3] Mattick J S.Type Ⅳ pili and twitching motility[J].Ann Rev Microbiol,2002,56:289-314.
[4] O'Toole G A,Kolter R.Flagellar and twitching motility are necessary for Pseudomonas aeruginosa biofilm development[J].Mol Microbiol,1998,30(2):295-304.
[5] Murray T S,Kazmierczak B I.Pseudomonas aeruginosaexhibits sliding motility in the absence of type Ⅳ pili and flagella[J].J Bacteriol,2008,190(8):2700-2708.
[6] Makin S A,Beveridge T J.The influence of A-band and B-band lipopolysaccharide on the surface characteristics and adhesion of Pseudomonas aeruginosa tosurfaces[J].Microbiology,1996,142(pt2):299-307.
[7] Singh P K.Iron sequestration by human lactoferrin stimulates P.aeruginosa surface motility and blocks biofilm formation[J].Biometals,2004,17(3):267-270.
[8] Patriquin G M,Banin E,Gilmour C,et al.Influence of quorum sensing and iron on twitching motility and biofilm formation in Pseudomonas aeruginosa[J].J Bacteriol,2008,190(2):662-671.
[9] Pamp S J,Tolker-Nielsen T.Multiple roles of biosurfactants in structural biofilm development by Pseudomonas aeruginosa[J].J Bacteriol,2007,189(6):2531-2539.
[10] Al-Tahhan R A,Sandrin T R,BodourA A,et al.Rhamnolipid-induced removal of lipopolysaccharide from Pseudomonas aeruginosa:Effect on cell surface properties and interaction with hydrophobic substrates[J].Appl Environ Microb,2000,66(8):3262-3268.
[11] Adam J,Pokorna M,Sabin C,et al.Engineering of PA-ⅡL lectin from Pseudomonas aeruginosa-Unravelling the role of the specificity loop for sugar preference[J].BMC Struct Biol,2007,7:36.
[12] Zegans M E,Wozniak D,Griffin E,et al.Pseudomonas aeruginosa exopolysaccharide Psl promotes resistance to the biofilm inhibitor polysorbate 80[J].Antimicrob Agents Chemother,2012,56(8):4112-4122.
[13] Ma L,Conover M,Lu H,et al.Assembly and developmentof the Pseudomonas aeruginosa biofilm matrix[J].PLoS Pathog,2009,5(3):e1000354.
[14] Ma L,Lu H,Sprinkle A,et al.Pseudomonas aeruginosa Psl is a galactose- and mannose-rich exopolysaccharide[J].J Bacteriol,2007,189(22):8353-8356.
[15] Ma L,Jackson K D,Landry R M,et al.Analysis of Pseudomonas aeruginosa conditional psl variants reveals roles for the psl polysaccharide in adhesion and maintaining biofilm structure postattachment[J].J Bacteriol,2006,188(23):8213-8221.
[16] Yang L,Hu Y F,Liu Y,et al.Distinct roles of extracellular polymeric substances in Pseudomonas aeruginosabiofilm development[J].Environ Microbiol,2011,13(7):1705-1717.
[17] Byrd M S,Sadovskaya I,Vinogradov E,et al.Genetic and biochemical analyses of the Pseudomonas aeruginosa Psl exopolysaccharide reveal overlapping roles for polysaccharide synthesis enzymes in Psl and LPS production[J].Mol Microbiol,2009,73(4):622-638.
[18] Mishra M,Byrd M S,Sergeant S,et al.Pseudomonas aeruginosa Psl polysaccharide reduces neutrophil phagocytosis and the oxidative response by limiting complement-mediated opsonization[J].Cell Microbiol,2012,14(1):95-106.
[19] Tseng B S,Zhang W,Harrison J J,et al.The extracellular matrix protects Pseudomonas aeruginosa biofilms by limiting the penetration of to bramycin[J].Environ Microbiol,2013,15(10):2865-2878.
[20] Billings N,Millan M,Caldara M,et al.The extracellular matrix component Psl provides fast-acting antibiotic defense in Pseudomonas aeruginosa biofilms[J].PLoS Pathog,2013,9(8):e1003526.
[21] Ma L,Wang S,Wang D,et al.The roles of biofilm matrix polysaccharide Psl in mucoidPseudomonas aeruginosa biofilms[J].FEMS Immunol Med Microbiol,2012,65(2):377-380.
[22] Colvin K M,Gordon V D,Murakami K,et al.The pel polysaccharide can serve a structural and protective role in the biofilm matrixof Pseudomonas aeruginosa[J].PLoS Pathog,2010,7(1):e1001264.
[23] Ghafoor A,Jordens Z,Rehm B H.Role of PelF in Pel polysaccharide biosynthesis in Pseudomonas aeruginosa[J].Appl Environ Microbiol,2013,79(9):2968-2978.
[24] Sakuragi Y,Kolter R.Quorum-sensing regulation of the biofilm matrix genes(pel）of Pseudomonas aeruginosa[J].J Bacteriol,2007,189(14):5383-5386.
[25] Yang L,Heng Z W,Wu H,et al.Polysaccharides serve as scaffold of biofilms formed by mucoid Pseudomonas aeruginosa[J].FEMS Immunol Med Microbiol,2012,65(2):366-376.
[26] Leid J G,Willson C J,Shirtliff M E,et al.The exopolysaccharide alginate protects Pseudomonas aeruginosa biofilm bacteria from IFN-gamma-mediated macrophage killing[J].J Immunol,2005,175(11):7512-7518.
[27] Vendeville A,Winzer K,Heurlier K,et al.Making'sensel' of metabolism:Autoinducer-2,LuxS and pathogenic bacteria[J].Nat Rev Microbiol,2005,3(5):383-396.
[28] Williams P,Winzer K,Chan W C,et al.Look who's talking:Communication and quorum sensing in the bacterial world[J].Philos Trans R Soc Lond Ser B Biol Sci,2007,362(1483):1119-1134.
[29] Winzer K,Hardie K R,Williams P.Bacterial cell-to-cell communication[J].Curr Opin Microbiol,2002,5(2):216-222.
[30] Irie Y,Borlee B R,O'Connor J R,et al.Self-produced exopolysaccharide is a signal that stimulates biofilm formation in Pseudomonas aeruginosa[J].Proc Natl Acad Sci USA,2012,109(50):20632-20636.
[31] Passador L,Cook J M,Gambello M J,et al.Expression of Pseudomonas aeruginosa virulence genes requires cell-to-cell communication[J].Science,1993,260(5111):1127-1130.
[32] Ochsner U A,Reiser J.Autoinducer-mediated regulation of rhamnolipid biosurfactant synthesis in Pseudomonas aeruginosa[J].Proc Natl Acad Sci USA,1995,92(14):6424-6428.
[33] Diggle S P,Cornelis P,Williams,P,et al.4-quinolone signalling in Pseudomonas aeruginosa:Old molecules,new perspectives[J].Int J Med Microbiol,2006,296(2):83-91.
[34] Davies D G,Parsek M R,Pearson J P,et al.The involvement of cell-to-cell signals in the development of a bacterial biofilm[J].Science,1998,280(5361):295-298.
[35] Frederix M,Downie A J.Quorum sensing:Regulating the regulators[J].Adv Microb Physiol,2011,58:23-80.
[36] Bjarnsholt T,Jensen P,Burmlle M,et al.Pseudomonas aeruginosa tolerance to tobramycin,hydrogen peroxide and ploymorphonuclear leukocytes is quorm-sensing dependent[J].Microbiology,2005,151(2):373-383.
[37] Folsom J P,Richards L,Pitts B,et al.Physiology of Pseudomonas aeruginsa in biofilms as revealed by transcriptome analysis[J].BMC Microbiol,2010,17(10):294.
[38] Kievit T R.Quorum sensing in Pseudomonas aeruginosa biofilms[J].Environ Microbiol,2009,11(2):279-288.
[39] Shrout J D,Chopp D L,Just C L,et al.The impact of quorum sensing and swarming motility on Pseudomonas aeruginosa biofilm formation is nutritionally conditional[J].Mol Microbiol,2006,62(5):1264-1277.
[40] Chicurel M.Bacterial biofilms and infections-slimebusters[J].Nature,2000,408(6810):284-286.
[41] Davies D.Understanding biofilm resistace to antibacterial agents[J].Nat Rev Drug Discov,2003,2(2):114-122.
[42] Ganguly K,Wu R,Ollivault-Shiflett M,et al.Design,synthesis,and a novel application of quorum-sensing agonists as potentialdrug-delivery vehicles[J].J Drug Target,2011,19(7):528-539.
[43] Klein T,Henn C,de Jong J C,et al.Identificationof small-molecule antagonists of the Pseudomonas aeruginosa transcriptionalregulator PqsR:Biophysically guided hit discovery and optimization[J].ACS Chem Biol,2012,7(9):1496-1501.
[44] Storz M P,Maurer C K,Zimmer C,et al.Validation of PqsD as an anti-biofilm target in Pseudomonas aeruginosa bydevelopment of small-molecule inhibitors[J].J Am Chem Soc,2012,134(39):16143-16146.
[45] Veesenmeyer J L,Hauser A R,Lisboa T,et al.Pseudomonas aeruginosa virulence and therapy:Evolving translational strategies[J].Crit Care Med,2009,37(5):1777-1786.
[46] Aiello D,Williams J D,Majgier-Baranowska H,et al.Discoveryand characterization of inhibitors of Pseudomonas aeruginosa typeⅢsecretion[J].J Sci Antimicrob Agents Chemother,2010,54(5):1988-1999.
[47] 谢林利,周密,陈勇川,等.黄芩苷、黄芩素抑制铜绿假单胞菌生物膜形成的研究[J].中国药房,2010,39:3651-3653.
[48] Zeng Z,Qian L,Cao L,et al.Virtual screening for novelquorum sensing inhibitors to eradicate biofilm formation of Pseudomonas aeruginosa[J].Appl Microbiol Biotechnol,2008,79(1):119-126.
[49] Jakobsen T H,van Gennip M,Phipps R K,et al.Ajoene,a sulfur-rich molecule from garlic,inhibits genescontrolled by quorum sensing[J].Antimicrobial Agents Chemother,2012,56(5):2314-2325.
[50] 林丽华,余加林,刘官信.大蒜素对铜绿假单胞菌生物膜形成的影响及结构定量化分析[J].中国微生态杂志,2012,24(1):26-30.
[51] 程惠娟,张昌峰,汪长中,等.鱼腥草素钠对铜绿假单胞菌生物被膜藻酸盐合成的影响[J].中成药,2013,35(3):455-461.
[52] Kapoor R,Wadman W M,Dohm M T,et al.Antimicrobial peptoids are effective against Pseudomonas aeruginosa biofilms[J].Antimicrobial Agents Chemother,2011,55(6):3054-3057.
[53] Pompilio A,Crocetta V,Scocchi M,et al.Potential novel therapeutic strategies in cystic fibrosis:Antimicrobial and anti-biofilm activity of natural and designed α-helical peptides against Staphylococcus aureus,Pseudomonas aeruginosa and Stenotrophomonas maltophilia[J].BMC Microbiol,2012,12(1):145.

Research Progress on Biofilms in Pseudomonas aeruginosa

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 2

Recommended Articles

Metrics

Comments

[1]	LI Xiaoyu, LI Ruoyu, WANG Xiao, WANG Lili, LI Jibin, LI Shuying, XU Yongping. Research Progress on the Influence of Filamentous Phages on Host Bacterial Adaptability [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(6): 2496-2506.
[2]	JIN Shengnan, SHU Huiping, ZHANG Dongxing, ZHAO Zelin, KANG Yuanhuan, ZHANG Lei, SHAN Xiaofeng, QIAN Aidong. Comparative Analysis of the Antimicrobial Activity of Lactobacillus Supernatants from Swine in Biofilm and Plankton [J]. China Animal Husbandry and Veterinary Medicine, 2019, 46(11): 3358-3369.