›› 2016, Vol. 43 ›› Issue (10): 2792-2796.doi: 10.16431/j.cnki.1671-7236.2016.10.042

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Rapid Extraction of Bacillus and Lactobacillus DNA by Microwave Heating

ZHOU Yan-yang, QIU Wan-si, TANG Jun-ni, TANG Cheng   

  1. College of Life Science and Technology, Southwest University for Nationalities, Chengdu 610041, China
  • Received:2016-03-29 Online:2016-10-20 Published:2016-10-28

Abstract:

The assay was aimed to investigate a fast,simple and efficient method with microwave heating for extracting DNA of Bacillus and Lactobacillus.Treated in a microwave oven at rated power (800 W) for a certain time (40 to 150 s),then centrifuged for supernatant.The supernatant was transferred to a sterile tube for PCR testing and agarose gel electrophoresis.Sequencing was carried out after determining the purity and yield.The results showed that heating for 40 to 150 s at 800 W microwave power conditions could effectively extract DNA of Bacillus and Lactobacillus.The obtained DNA quality was good (D260 nm/D280 nm was 1.8 to 2.1).The DNA concentration in line with the PCR testing requirements;Sequencing results was satisfied with the routine molecular biological research.The microwave extraction method had simple,fast and efficient features,and a wide range of practical,which provided a simple means for rapid molecular assay.

Key words: microwave heating; DNA extraction; Bacillus; Lactobacillus

CLC Number: