牦牛HOXA1基因的时空表达及其对机体调控机制的研究

doi:10.16431/j.cnki.1671-7236.2016.09.007

Abstract

Abstract:

The aim of study was to obtain the yak HOXA1 gene sequence and related bioinformatics information,and analyze its expression spectrum and temporal expression profiles for providing related information of the formation of multiple vertebrae and its mechanism.RT-PCR technology was applied to clone the yak HOXA1 gene,semi-quantitative RT-PCR technique was used to detect the gene expression level in multiple organisms,and Real-time quantitative PCR technique was used to detect the gene expression level in the organisms in different development periods.The results showed that the obtained HOXA1 gene was 885 bp including the ORF of 870 bp,encoding 290 amino acids.The yak HOXA1 gene shared a homology of 75.4% to 98.1% with ordinary cow,wild yak,human,wild boar,horse,mice,chimpanzees,jungle fowl and wild boar.There were different expression level of HOXA1 gene in heart,liver,spleen,lung,kidney,large intestine,small intestine,muscle,stomach,ovaries,uterus,fallopian tubes,breast and testicular tissue.With the growth of the yak,HOXA1 gene expression level in tissue also increased.

Key words: yak; HOXA1 gene; cloning; sequence analysis; tissue expression; temporal expression

CLC Number:

ZHANG Ya-nan, SHI Xian, XIONG Xian-rong, LAN Dao-liang, LI Jian. Expression Pattern of HOXA1 Gene and Its Putative Regulating Mechanism of Yak[J]. , 2016, 43(9): 2265-2271.

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Expression Pattern of HOXA1 Gene and Its Putative Regulating Mechanism of Yak

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