京海黄鸡中HOXC10基因表达规律及其生物信息学分析

doi:10.16431/j.cnki.1671-7236.2016.07.007

Abstract

Abstract:

HOX genes express in nearly all eukaryotic cells such as yeast and human.To study tissue expression and bioinformatics of HOXC10 gene,the samples of heart,liver,spleen,lung,kidney,chest muscle,leg muscle,hypothalamus and ovary tissues were collected from 6 Jinghai Yellow chicken with 8-week-old to analyze the expression pattern of HOXC10 gene in different tissues by qRT-PCR method,and the different expression level were detected in ovary of 300 days old chickens in high yield group and low yield group.The bioinformatics of HOXC10 gene were analyzed by a variety of online softwares of Gallus gallus.The result showed that the expression level of HOXC10 gene in leg muscle was extremely significantly higher than that in other tissues of Jinghai Yellow chicken (P < 0.01);And it's expression level in low yield group was significantly higher than that in high yield group (P < 0.05);HOXC10 gene of Gallus gallus shared 77.4%,94.8%,98.6%,68.3%,67.9%,66.0%,59.0%,68.1% and 79.4% with Meleagris gallopavo,Columba,Anas platyrhynchos,Homo sapiens,Mus musculus,Bos taurus,Sus scrofa,Capra aegagrus hircus and Xenopus laevis,respectively;HOXC10 gene encoded 354 amino acids in which serine was the most abundant;There were 24 potential phosphorylation sites and a HD in 280 to 342 position of amino acid sequence;The subcellular of the encoding protein was mainly located in the cell nucleus,and it had no cross membrane structure.HOXC10 gene might play an important role in regulation network of growth and reproduction in Jinghai Yellow chicken.

Key words: HOXC10 gene; Jinghai Yellow chicken; tissue expression analysis; bioinformatics

CLC Number:

Q786

ZHANG Xiang-qian, DONG Xin-long, LING Jiao-jiao, HAN Kun-peng, ZHANG Tao, WANG Jin-yu, WANG Yong-juan. Tissue Expression and Bioinformatics Analysis of HOXC10 Gene in Jinghai Yellow Chicken[J]. , 2016, 43(7): 1702-1708.

References

[1] Shah N,Sukumar S.The Hox genes and their roles in oncogenesis[J].Nature Reviews Cancer,2010,10:361-371.
[2] Krumlauf R.Hoh genes in vertebrate development[J].Cell,1994,78(2):191-201.
[3] Foronda D,De Navas L,Garaulet D,et al.Function and specificity of Hox genes[J].The International Journal of Developmental Biology,2009,53:1409-1419.
[4] Jiang X,Russo I H,Russo J.Alternately spliced luteinizing hormone/human chorionic gonadotropin receptor mRNA in human breast epithelial cells[J].International Journal of Oncology,2002,20(4):735-738.
[5] Moucadel V,Totaro M S,Dell C D,et al.The homeobox gene Cdx1 belongs to the p53-p21 (WAF)-Bcl-2 network in intestinal epithelial cells[J].Biochemical and Biophysical Research Communications,2002,297(3):607-615.
[6] Drabkin H A,Parsy C,Ferguson K,et al.Quantitative HOX expression in chromosomally defined subsets of acute myelogenous leukemia[J].Leukemia,2002,16(2):186-195.
[7] 杜玲,刘刚,陆健,等.高通量测序技术的发展及其在生命科学中的应用[J].中国畜牧兽医,2014,41(12):109-116.
[8] 张跟喜.边鸡遗传多样性及Moystatin基因对生长和繁殖性状的遗传效应研究[D].扬州:扬州大学,2010.
[9] Denans N,Iimura T,Pourquié O.Hox genes control vertebrate body elongation by collinear Wnt repression[J].Elife,2015,4:e04379.
[10] Gouveia A,Marcelino H M,Gonçalves L,et al.Patterning in time and space:HoxB cluster gene expression in the developing chick embryo[J].Cell Cycle,2015,14(1):135-145.
[11] Feng X,Li T,Liu Z,et al.HOXC10 up-regulation contributes to human thyroid cancer and indicates poor survival outcome[J].Molecular Biosystems,2015,11(11):2946-2954.
[12] Ansari K I,Hussain I,Kasiri S,et al.HOXC10 is overexpressed in breast cancer and transcriptionally regulated by estrogen via involvement of histone methylases MLL3 and MLL4[J].Journal of Molecular Endocrinology,2012,48(1):61-75.
[13] Gabellini D,Colaluca I N,Vodermaier H C,et al.Early mitotic degradation of the homeoprotein HOXC10 is potentially linked to cell cycle progression[J].The EMBO Journal,2003,22(14):3715-3724.
[14] 陈绍军.无蹼壁虎尾的形态结构与其自截断尾和断尾活动的研究[J].中药材,1992,15(11):10-13.
[15] Carlson M R,Komine Y,Bryant S V,et al.Expression of Hoxb13 and Hoxc10 in developing and regenerating axolotl limbs and tails[J].Dev Biolo,2001,229:396-406.
[16] Christen B,Beck C W,Lombardo A,et al.Regeneration-specific expression pattern of three posterior Hox genes[J].Dev Dyn,2003,226:349-355.
[17] 李宇,熊显荣,兰道亮,等.麦洼牦牛HOXA10基因的克隆、原核表达及组织表达谱[J].西北农业学报,2013,22(7):7-11.
[18] Blitek A,Kaczmarek M M,Kiewisz J,et al.Endometrial and conceptus expression of HOXA10,transforming growth factor beta1,leukemia inhibitory factor,and prostaglandin H synthase-2 in early pregnant pigs with gonadotropin-induced estrus[J].Domest Anim Endocrinol,2010,38(4):222-234.
[19] Crow K D,Stadler P F,Lynch V J,et al.The"fish-specific"Hox cluster duplication is coincident with the origin of teleosts[J].Molecular Biology and Evolution,2006,23:121-136.

Tissue Expression and Bioinformatics Analysis of HOXC10 Gene in Jinghai Yellow Chicken

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