猪肌分化因子1基因启动子区多态性及生物信息学研究

doi:10.16431/j.cnki.1671-7236.2016.05.028

Abstract

Abstract: In order to reveal the polymorphism of MyoD1 gene promoter in pigs, three pig breeds(wild boar Xiang pigs, commercial crossbred pigs and Guizhou Zongdi Hua pigs) were selected as the experimental subjects.By constructing DNA pool and using the technology of direct sequencing to screen the single nucleotide polymorphism on MyoD1 gene promoter region in three groups.Variety bioinformatics softwares were used to predict the core region of the promoter, transcription factors binding sites and CpG island.The results showed that the experiment had totally found three SNPs in MyoD1 gene, among these SNPs, A-39G was located in 5'UTR, T+150C and C+227G were located in exon 1.It was identified that various new transcription factors binding sites emerged and some significant transcription factors binding sites disappeared at the region nearby A-39G site.The software of CpG island searcher indicated that SNP mutation could change the range of CpG island and the content of GC.Therefore, A-39G site locating MyoD1 gene 5'UTR had an important effect on regulating the functional element of promoter.

Key words: MyoD1 gene; SNP; promoter; bioinformatics

CLC Number:

S813.3

ZHANG Xiong, ZHANG Yong, CHEN Xiang, LI Jun, BAI You, CHEN Cun-nian, LI Dong-guang. Study on Polymorphisms and Bioinformatics of MyoD1 Gene Promoter Region in Pig[J]. , 2016, 43(5): 1308-1315.

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Study on Polymorphisms and Bioinformatics of MyoD1 Gene Promoter Region in Pig

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