›› 2015, Vol. 42 ›› Issue (10): 2753-2761.doi: 10.16431/j.cnki.1671-7236.2015.10.036

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Diagnosis and Treatment of Porcine Epidemic Diarrhea Co-infecting with Porcine Circovirus Disease

LIU Yang1,2, KU Xu-gang1,2, LIU Xiao-li2, HE Qi-gai1,2   

  1. 1. State Key Laboratory of Agriculture Microbiology, Wuhan 430070, China;
    2. College of Animal Science and Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, China
  • Received:2015-03-20 Online:2015-10-20 Published:2015-10-23

Abstract: In February 2014,piglets of a certain 100 sows,fed in a large-scale pig farm which had 2 000 sows,died acutely and densely in one week with vomit,emaciation and lethal watery diarrhea after sudden snowy weather.By field survey,pathological anatomy,histopathological observation,immunohistochemistry,reverse transcription-polymerase chain reaction (RT-PCR),sequencing and enzyme-linked immune sorbent assay (ELISA),porcine epidemic diarrhea virus (PEDV),co-infecting with porcine circovirus 2 (PCV2),was confirmed to be the major agent.By adopting measures,including emergency vaccination of sows,egg yolk antibody oral inoculation of piglets,and insulation and disinfection,the disease was successfully and speedily controlled.

Key words: porcine epidemic diarrhea virus; RT-PCR; S gene; diagnose

CLC Number: