副猪嗜血杆菌丝氨酸蛋白酶基因的克隆及生物信息学分析

doi:10.16431/j.cnki.1671-7236.2015.10.018

Abstract

Abstract: In order to evaluate whether Haemophilus parasuis espP gene could use as a vaccine candidate antigen or not,the espP gene was amplified by PCR using the specific primers that designed according to corresponding sequence getting form GenBank (accession No.:HAPS_1381) and then sent to sequence.After translating the DNA sequence into amino acid sequence,the signal peptide,hydrophilic region,linear B cell epitopes and 3D structure of Hps espP protein were predicted using multiple bioinformatics analysis.The results showed that the full length of Hps espP gene was 2 343 bp,encoding 781 aa.The first 23 aa were predicted as signal peptide.There were 12 hydrophilic regions and 12 B cell epitopes in the Hps espP protein.The 3D structure of C-terminal of the protein was consisted of a monomeric β-barrel containing 12 β-strands and a central pore.The study laid the foundation for further development of molecular vaccines based on espP protein against Hps infection.

Key words: Haemophilus parasuis; espP; clone; bioinformatics

CLC Number:

S852.61⁺2

YOU Jin-zhou, ZHANG Rui-zhen, QIU Jian-long, XIE Min, HU Ling-ying. Cloning and Bioinformatics Analysis of espP Gene of Haemophilus parasuis[J]. , 2015, 42(10): 2631-2635.

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Cloning and Bioinformatics Analysis of espP Gene of Haemophilus parasuis

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