鸭坦布苏病毒E基因在昆虫细胞中的分泌表达及免疫原性与保护性研究

doi:10.16431/j.cnki.1671-7236.2015.10.010

Abstract

Abstract: Duck Tembusu virus (DTMUV) was newly emerged which caused severe egg-drop syndrome in duck.Here,we constructed the recombinant baculovirus by honeybee melittin signal peptide (Mels) which expressed E protein of DTMUV JSXZ strain,and its immunogenicity and protective efficacy were investigated in duck infection model.Western blotting and indirect immunofluorescence assay (IFA) showed E protein was effectively secretory expressed in supernatant of Sf9 insect cells.Indirect ELISA result showed E protein could induce high levels of anti-E IgG in the sera.MTT result showed that E protein could stimulate the proliferation of T lymphocytes.Moreover,the protection rates were 80% in 0.3 mL group and 100% in both 0.6 mL and 0.8 mL groups,respectively,whereas,all ducks in PBS control group showed the typical clinical signs with 3 died post challenge.These results laid a foundation for the study of DTMUV subunit vaccine.

Key words: duck Tembusu virus (DTMUV); E protein; baculovirs; secretory expression; immunogenicity; protective efficacy

CLC Number:

S852.65

WANG Shan-hui, XIE Jin-wen, LV Yan-zhi, LI Yun-long. Secretory Expression of DTMUV E Gene in Insect Cells and Study on its Immunogenicity and Protective Efficacy[J]. , 2015, 42(10): 2580-2586.

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Secretory Expression of DTMUV E Gene in Insect Cells and Study on its Immunogenicity and Protective Efficacy

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