不同辐照剂量对蜂球囊菌杀灭效果的影响

doi:10.16431/j.cnki.1671-7236.2015.04.038

Abstract

Abstract: In order to obtain the lowest irradiation dose on effectively killing Ascosphaera apis, our study took Ascosphaera apis as the research object, and explored the killing effect of different irradiation dose on the Ascosphaera apis. Also, we hoped to provide a reference basis for Co60γ irradiating bee feed (especially for bee pollen). During the study, we had used bacteria-purification technology to obtaine Ascosphaera apis from cretaceous disease (bee chalkbrood diseases) larvae, and combined with morphology, lactic acid phenol medan dyeing and 5.8S rDNA sequence analysis technology to identificate this Ascosphaera apis. In addition, we preparated different levels of Ascosphaera apis spore suspension, and added them to 3 d larvae feed to test the half lethal dose (LC₅₀). Behind, we added LC₅₀ level of spore suspension into larvae diet, and then adopted different irradiation dose irradiation diet, finally fed larvae with that treated diets. The results showed that the isolated and purified fungal pathogen, which got from bee chalkbrood disease larvae, were bee balloon bacteria, identified by morphological and molecular biology. Furthermore, under the condition of indoor artificial breeding, the LC₅₀ of bacteria Ascosphaera apis on larvae was 9.5×10⁴/mL, and the effectively killing dose was 7.0 kGy. There was significant difference between larvae prevalence and the irradiation group (P<0.05). From this we could be sure that the minimum effective radiation dose of bee feed, which was added LC₅₀ balloon fungus spores was 7.0 kGy.

Key words: Ascosphaera apis; irradiation dose; bees feed; chalkbrood disease

CLC Number:

S896

DONG Wen-bin, LIU Feng, LI Xiao, XU Bao-hua. Effect of Different Irradiation Dosage on the Killing of Ascophaera apis[J]. , 2015, 42(4): 1021-1027.

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Effect of Different Irradiation Dosage on the Killing of Ascophaera apis

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