应用PCR-DGGE分析鸭场土壤细菌群落结构

doi:10.16431/j.cnki.1671-7236.2015.01.010

Abstract

Abstract: To investigate the diversity of bacterial community structure in soil sample of 8 duck farms in Sansui Guizhou province, total DNA of the samples were extracted with kit, the bacterial 16S rDNA V3 variable sequence was amplified by PCR, the amplified sequence were separated by denaturing gradient gel electrophoresis (DGGE) technique, 43 bands were showed, number 7, 8 were relatively small.The DGGE fingerprint was analyzed by software Quantity One, and showed that the same duck farm similarities existed difference, while the similarity were higher than 50% of the majority.Results showed that the similarities between the sequences and sequences in GenBank were 90% to 100%, including α-Proteobacteria, β-Proteobacteria, Bacteroidetes, Actinobacteria, Acidobacteria and Firmicutes.

Key words: denaturing gradient gel electrophoresis; duck farm; soil; bacterial community structure

CLC Number:

S154.3

LI Shi-jing, JI Xin-qin, WEN Ming, CHEN Jia-qi, LIU Ting-jiang. Analysis of Bacterial Community Structure in Soil Sample of Duck Farm by PCR-DGGE[J]. , 2015, 42(1): 67-73.

References

[1] Muyzer G,de Waal E C,Uitterlinden A G.Profiling of complex microbial populations by denaturing gradient gel electrophoresis analysis of polymerase chain reaction-amplified genes coding for 16S rRNA[J].Applied and Environmental Microbiology,1993,59(3):695-700.
[2] 马悦欣,Holmstrm C,Webb J,等.变性梯度凝胶电泳(DGGE)在微生物生态学中的应用[J].生态学报,2003,23(8):1561-1569.
[3] Casamay or E O,Schfer H,Baneras L,et al.Identification of and spatiotemporal differences between microbial assemblages from two neighboring sulfurous lakes:Comparison by microscopy and denaturing gradient gel electrophoresis[J].Applied and Environmental Microbiology,2000,66(2):499-508.
[4] Bano N,Hollibangh J T.Phylogenetic composition of bacterioplanton assemblages from Arctic ocean[J].Applied and Environmental Microbiology,2002,68(2):505-518.
[5] 邢薇,左剑恶,孙寓姣,等.利用FISH和DGGE对产甲烷颗粒污泥中微生物种群的研究[J].环境科学,2006,27(11):2268-2272.
[6] 段学军,闵航.镉胁迫下稻田土壤微生物基因多样性的DGGE分子指纹分析[J].环境科学,2004,25(5):122-126.
[7] 李沛军,孔保华,郑冬梅.PCR-DGGE技术在肉品微生物生态学中的应用[J].食品科学,2012,33(19):338-343.
[8] 吴高峰,李文刚,高卫科,等.PCR-DGGE的原理及在动物肠道菌群分析中的应用[J].中国畜牧兽医,2008,35(6):37-37.
[9] 陈静,马松成,毛华明,等.DGGE/TGGE技术在微生物生态学中的应用[J].中国畜牧兽医,2006,33(11):47-50.
[10] Karita S,Nakayama K,Goto M,et al.A novel cellulolytic,anaerobic,and thermophilic bacterium,Moorella sp strain F21[J].Bioscience,Biotechnology and Biochemistry,2003,67(1):183-185.
[11] Ampe F,Ben Omar N,Moizan C,et al.Polyphasic study of the spatial distribution of microorganisms in mexican pozol,a fermented maize dough,demonstrates the need for cultivation-independent methods to investigate traditional fermentations[J].Applied and Environmental Microbiology,1999,65:5464-5473.
[12] 王岳坤,洪葵.红树林土壤细菌群落16S rDNA V3片段PCR产物的DGGE分析[J].微生物学报,2005,45(2):201-204.
[13] 李丹.不同土地利用方式对土壤性质及细菌群落结构的影响[D].哈尔滨:东北林业大学,2011.
[14] 陈亮.规模化肉种鸭场环境卫生指标菌的检测及与种鸭生产性能相关性研究[D].雅安:四川农业大学,2008.
[15] 李楠.养殖场空气中细菌分布及耐药性研究[D].北京:中国人民解放军军事医学科学院,2011.
[16] 何涛,张海军,武书庚,等.PCR-DGGE分子技术及其在畜牧业中的应用[J].中国畜牧兽医,2008,35(7):36-39.
[17] Seghers D,Wittebolle L,Top E M,et al.Impact of agricultural practices on the Zea mays L.endophytic community[J].Appl Environ Microbiol,2004,70:1475-1482.
[18] 周小奇,王艳芬,蔡莹.内蒙古典型草原细菌群落结构的PCR-DGGE检测[J].生态学报,2007,27(5):1684-1689.

Analysis of Bacterial Community Structure in Soil Sample of Duck Farm by PCR-DGGE

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