布鲁氏菌LPS对小鼠巨噬细胞中NLRP3炎症小体转录水平的影响

doi:10.16431/j.cnki.1671-7236.2015.01.002

›› 2015, Vol. 42 ›› Issue (1): 9-16.doi: 10.16431/j.cnki.1671-7236.2015.01.002

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Effect of Brucella LPS on NLRP3-inflammasome Transcriptional Level of Mouse Macrophages

YUAN Sha, CHEN Peng-bo, CHEN Chuang-fu

College of Animal Science and Technology, Shihezi University, Shihezi 832003, China

Received:2014-07-25 Online:2015-01-20 Published:2015-02-06

Abstract

Abstract: In order to find out the impact of Brucella LPS on macrophages NLRP3-inflammasome, the mouse macrophages were infected by the different concentrations of LPS which extracted from Brucella 2308, RB51 and △WbkA to explore the changes of NLRP3-inflammasome related genes, ASC, caspase1, IL1-β, IL18 at transcription levels using Real-time PCR.The results showed RB51 LPS and △WbkA LPS regulating NLRP3-inflammasome related genes transcription relied on concentrations, and concentrations of 2308 LPS had little effect on NLRP3-inflammasome related genes transcription; And under the same concentration, RB51 LPS and 2308 LPS better regulated Caspase1, IL1-β, IL18 transcriptions than △WbkA LPS.

Key words: Brucella LPS; mouse macrophage; NLRP3-inflammasome

CLC Number:

YUAN Sha, CHEN Peng-bo, CHEN Chuang-fu. Effect of Brucella LPS on NLRP3-inflammasome Transcriptional Level of Mouse Macrophages[J]. , 2015, 42(1): 9-16.

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Effect of Brucella LPS on NLRP3-inflammasome Transcriptional Level of Mouse Macrophages

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