苦参碱对多重耐药表皮葡萄球菌整合子intⅠ和dfrA的影响

doi:10.11843/j.issn.1671-7236.2015.03.009

Abstract

Abstract: To explore the effects of matrine on intⅠ and dfrA of integron in clinical multidrug resistant Staphylococcus epidermidis isolates.The K-B method was used to screen multidrug resistant isolates.The broth dilution method was applied to measure the minimum inhibitory concentration (MIC) and the best reversal condition.The ordinary PCR was applied to detect effect of matrine on intⅠ and dfrA gene sequences and Real-time PCR was applied to detect effect of matrine on intⅠ and dfrA mRNA expression levels.The ordinary PCR results verified that matrine had no effects on int Ⅰ and dfrA gene sequences.The Real-time PCR results demonstrated that int Ⅰ gene expression was down-regulated in presence of matrine rather than dfrA gene.Integron mRNA expression quantity of Staphylococcus epidermidis was decreased by matrine in vivo.

Key words: matrine; Staphylococcus epidermidis; integron; intⅠ; dfrA

CLC Number:

S852.61⁺1

JIA Fang, YANG Jiang-liu, XING Li-qun, LIU Wen-hui. Effects of Matrine on intⅠ and dfrA of Integron in Clinical Multidrug Resistant Staphylococcus epidermidis Isolates[J]. , 2015, 42(3): 563-568.

References

[1] Bennett P M.Plasmid encode antibiotic resistance:Acquisition and transfer of antibiotic resistances in bacteria[J].British Journal of Pharmacology,2008,153(Supp1):347-357.

[2] 刘衡川,扈庆华,兰全学,等.致泌尿系统感染中大肠埃希菌Ⅰ类整合子的研究[J].中国卫生检验杂志,2006,16(3):278-280.

[3] Akram M,Shakil S,Khan A U.Prevalence of integrons,bla_CTX-M and bla_TEM resistance markers among ESBL-producting uropathogenic Escherichia coli isolates:First report of genomic bla_CTX-M from India[J].Journal of Chemotherapy,2011,23(3):131-134.

[4] 高昂,于红.产超广谱β-内酰胺酶细菌中Ⅰ类整合子的研究进展[J].微生物学通报,2013,40(11):2107-2114.

[5] Martinez-Freijo P,Fluit A C,Schmitz F J,et al.Class Ⅰ integrons in Gram-negative isolates from different European hospitals and association with decreased susceptibility to multiple antibiotic compounds[J].J Antimicrob Chemother,1998,42(6):689-696.

[6] DÍaz-Mejí;a J,Amíbile-Cuevas C F,Rosas I,et al.An analysis of the evolutionary relationships of integron integrases,with emphasis on the prevalence of class Ⅰ integrons in Escherichia coli isolates from clinical and environmental origins[J].Microbiology,2008,154(1):94-102.

[7] 邢立群,贾芳.甘草次酸联合抗结核药对结核杆菌活性研究[J].中国畜牧兽医,2014,41(4):160-164.

[8] 李鲁明,袁晓燕,王明义,等.整合子—基因盒系统与细菌耐药机制的研究进展[J].中国微生态学杂志,2014,26(2):246-248.

[9] 李柳琴,饶富顺.表皮葡萄球菌112株耐药性分析[J].检验医学与临床,2013,10(6):739-740.

[10] Zhou X,Jia F,Liu X,et al.Total alkaloids from Kudouzi increase susceptibility of extended-spectrum-lactamases-producing Escherichia coli isolates to cefotaxime and ceftazidime[J].International Journal of Antimicrobial Agents,2013,19(12):945-952.

[11] 张英,祁鑫,朱小云,等.氧化苦参碱对人乳腺癌MCF-7 细胞的生长抑制作用及其机制研究[J].现代肿瘤医学,2014,22(3):494-497.

[12] 任玲玲,关立增.连翘对大肠埃希菌多重耐药基因AcrA的影响研究[J].动物医学进展,2008,29(5):43-45.

Effects of Matrine on intⅠ and dfrA of Integron in Clinical Multidrug Resistant Staphylococcus epidermidis Isolates

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 7

Recommended Articles

Metrics

Comments

[1]	CHEN Yuxiao, LI Huanjuan, XI Xiaohua, HAO Jie, ZHOU Degang, SI Hongbin. Study on Extraction Process of Jiawei Pingwei Oral Liquid [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(6): 2414-2422.
[2]	GE Qiang, MA Dongxin, ZHOU Yuqing, CHEN Huaijun, YUAN Jingzhi, LI Xun, WANG Xiaoye. Isolation,Identification and Biological Characteristics of Proteus mirabilis of Swine [J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(5): 1804-1815.
[3]	YUAN Xiaomeng, ZHAO Xiaonan, LI Lulu, ZHANG Qing, HU Ming, LUO Yanbo, ZHANG Yin, QI Jing, LIU Yuqing. Isolation,Identification and Drug Resistance Analysis of E.coli from Mastitis Milk in Shandong Province [J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(1): 312-323.
[4]	LIN Xi, LYU Shiming, TAN Aijuan, LUO Zhiqian, YANG Ruizhi, ZHENG Ruqing, ZHOU Ling. Correlation Between Transposon and Integron Carrying Type and Multidrug Resistance Spectrum of Escherichia coli [J]. China Animal Husbandry and Veterinary Medicine, 2020, 47(5): 1547-1559.
[5]	WANG Hai-sheng, HU Rui-ping, GAO Yu-min, YANG Zhong, DENG Xiu-ling, ZHANG Jian-yu, WU Rui-bing, HUHE Bateer. Study on Molecular Characterization of Class Ⅰ Integron and Integron-associated Antimicrobial Resistance in Escherichia coli from Beef Cattle [J]. , 2014, 41(9): 63-68.
[6]	ZHAO Qing-mei, YU Yong-tao, MA Yong-ping. Preliminary Analysis on the Alkaloids of Swainsona salsula [J]. , 2012, 39(11): 135-139.
[7]	LI Chengzhong;WANG Hongning;HUANG Yong;MA Menggen;WU Qi;YANG Yunfei;XIE Tao;LIU Ping. Study on Detection I Integron of Multidrug Resistace Pathogenic Salmonella from Chicken [J]. , 2007, 34(1): 119-123.