›› 2009, Vol. 36 ›› Issue (7): 88-92.

• 生物技术 • 上一篇    下一篇

牦牛Hepcidin 基因的克隆与序列分析

厍睿1,2,潘和平1,阎萍2,裴杰2   

  1. (1.西北民族大学生命科学与工程学院, 兰州 730030; 2.中国农业科学院兰州畜牧与兽药研究所, 兰州 730050)
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2009-07-20 发布日期:2009-07-20
  • 通讯作者: 裴杰

Clone and Sequence Analysis of Yak’s Hepcidin Gene

SHE Rui1,2, PAN He-ping1, YAN Ping2, PEI Jie2   

  1. (1.Northwest University for Nationalties, Life Science and Engineering Institute, Lanzhou 730030, China;2.China Academy of Agricultural Sciences, Institute of Animal Science and Veterinary Parmaceutics, Lanzhou 730050, China)
  • Received:1900-01-01 Revised:1900-01-01 Online:2009-07-20 Published:2009-07-20
  • Contact: PEI Jie

摘要: 根据GenBank中公布的普通牛Hepcidin基因序列(登录号为XM-589792.3)设计1对特异性引物。采用RT-PCR技术从牦牛肝脏组织总RNA中扩增出Hepcidin基因的编码序列并进行测序分析,同时构建Hepcidin蛋白物种进化树。结果显示,经克隆获得牦牛Hepcidin基因322 bp的cDNA序列(GenBank登录号为EU863791),含289 bp的开放阅读框,编码82个氨基酸,包括22个氨基酸的信号肽;预测Hepcidin蛋白分子质量和等电点分别为8.88 ku 和 9.24;与已知普通牛Hepcidin序列的同源性达 99%,不同物种Hepcidin蛋白具有高度的保守性,Hepcidin蛋白物种进化树符合物种进化规律。本研究为Hepcidin基因cDNA 全长克隆及基因功能研究奠定了重要基础。

关键词: 牦牛; Hepcidin基因; 克隆; 序列分析

Abstract: A pair of primers was designed and synthesized according to the bovine Hepcidin gene sequence in GenBank. Coding sequence of Hepcidin gene was amplified by RT-PCR technology from total RNA of yak’s live, the sequence of Hepcidin was analysed and phylogenetic tree of Hepcidin protein was constructed. The results showed that a 322 bp cDNA sequence of Hepcidin gene was cloned, including 289 bp open reading frames, encoding 82 amino acids which contained 22 amino acids coded signal peptide. Forecast for protein molecular weight and isoelectric points, was 8.88 ku and 9.24 respectively. This gene region had high conservation in the bovine species, compared with the ordinary cow, the homology of Hepcidin sequence was up to 99 percent.The phylogenetic tree conformed to the laws of species evolution. The research laid an important foundation on the full-length cDNA gene cloning and study.

Key words: yak; Hepcidin gene; clone; sequence analysis

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