黄曲霉毒素B1与M1对小鼠肠道细菌多样性的影响

doi:10.16431/j.cnki.1671-7236.2019.02.013

《中国畜牧兽医》 ›› 2019, Vol. 46 ›› Issue (2): 433-441.doi: 10.16431/j.cnki.1671-7236.2019.02.013

黄曲霉毒素B₁与M₁对小鼠肠道细菌多样性的影响

张牧臣, 郑楠, 赵圣国, 张养东, 李松励, 王加启

中国农业科学院北京畜牧兽医研究所, 农业部奶及奶制品质量安全控制重点实验室, 农业部奶产品质量安全风险评估实验室(北京), 农业部奶及奶制品质量监督检验测试中心(北京), 动物营养学国家重点实验室, 北京 100193

收稿日期:2018-06-22 出版日期:2019-02-20 发布日期:2019-02-20
通讯作者: 王加启 E-mail:jiaqiwang@vip.163.com
作者简介:张牧臣(1991-),男,硕士,研究方向:动物营养与饲料科学,E-mail:muchen805215099@163.com
基金资助:
国家现代农业产业技术体系建设专项（nycytx-04-01）；中国农业科学院科技创新工程项目（ASTIPIAS12）；国家自然科学基金项目（31501399）；公益性行业（农业）科研专项（201403071）；动物营养学国家重点实验室自主课题（2004DA125184G1611）

Effects of Aflatoxin B₁ and M₁ on Intestinal Bacterial Diversity in Mice

ZHANG Muchen, ZHENG Nan, ZHAO Shengguo, ZHANG Yangdong, LI Songli, WANG Jiaqi

Ministry of Agriculture-Key Laboratory of Quality & Safety Control for Milk and Dairy Products, Ministry of Agriculture-Laboratory of Quality and Safety Risk Assessment for Dairy Products, Ministry of Agriculture-Milk and Dairy Product Inspection Center, State Key Laboratory of Animal Nutrition, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, China

Received:2018-06-22 Online:2019-02-20 Published:2019-02-20

摘要/Abstract

摘要：

试验旨在研究黄曲霉毒素B₁与M₁（AFB₁与AFM₁）对小鼠肠道细菌多样性的影响。选取体况良好的4周龄ICR（CD-1）雄性小鼠40只，随机分成4组，每组10个重复，每个重复1只小鼠，其中AFB₁组小鼠每只每天灌胃0.3 mg/kg体重AFB₁，AFM₁组小鼠每只每天灌胃3.0 mg/kg体重AFM₁，AFB₁+AFM₁组每只每天灌胃AFB₁与AFM₁的混合溶液，其中AFB₁终浓度0.3 mg/kg体重，AFM₁终浓度3.0 mg/kg体重，以上3组毒素溶剂均为1.0%二甲基亚砜水溶液。对照组小鼠每只每天灌胃1.0%二甲基亚砜水溶液。每只灌胃剂量均为200 μL，每天09：00灌胃一次，连续灌胃28 d。灌胃结束后，处死并解剖小鼠，收集结肠内容物，采用16S rRNA测序的方法对肠内容物细菌多样性进行测序分析。结果显示：在细菌群落的门水平、科水平及属水平，与对照组相比，3个毒素处理组小鼠肠道内容物细菌优势菌群均未发生明显排序变化（P>0.05），但不同的毒素处理仍造成了不同分类水平下细菌菌群丰度的显著变化：与对照组相比，AFB₁组及联合灌胃组小鼠肠内致病菌或条件致病菌，如Facklamia、Staphylococcus、Corynebacterium属细菌丰度显著升高（P<0.05）；而AFM₁组与对照组相比未见显著差异（P>0.05）。综合试验结果，AFB₁单独作用或与AFM₁联合作用可诱导小鼠肠道内致病菌或条件致病菌细菌增殖，改变肠道健康微生物区系，损伤肠道微生物屏障功能。

关键词: 黄曲霉毒素B₁(AFB₁); 黄曲霉毒素M₁(AFM₁); 细菌多样性

Abstract:

To study the effect of aflatoxin B₁ and M₁ on intestinal bacterial diversity in mice,40 ICR male mice (4 weeks) were randomized into four groups of 10 each and received an oral administration of 0.3 mg/(kg·BW) AFB₁,3.0 mg/(kg·BW) AFM₁,mixture of AFB₁ and AFM₁ (0.3 mg/(kg·BW) AFB₁,3.0 mg/(kg·BW) AFM₁) and vehicle (1.0% DMSO) for 28 days (once a day at 9:00 am).Mice were executed after gavage,and intestinal contents were collected.The bacterial diversity of intestinal contents was analyzed by 16S rRNA sequencing.The results showed that compared with control group,there was no significant change in the dominant bacteria microflora in three experimental groups,regardless of the phylum level,family level or genus level(P>0.05).However,different toxin treatments still resulted in significant changes in the abundance of different bacteria microflora:Pathogenic bacteria or opportunistic pathogens abundance in the AFB₁ treatment and its combined treatment group with AFM₁,such as Facklamia,Staphylococcus and Corynebacterium were significantly increased compared with the control group (P<0.05),but not for AFM₁ group.Comprehensive analysis showed that individual AFB₁ or combined with AFM₁ could increase the abundance of pathogenic bacteria or opportunistic pathogens,and could change the healthy intestinal bacterial diversity,damage the function of intestinal microbial barrier.

Key words: aflatoxin B₁(AFB₁); aflatoxin M₁(AFM₁); bacterial diversity

中图分类号:

S859.87

张牧臣, 郑楠, 赵圣国, 张养东, 李松励, 王加启. 黄曲霉毒素B₁与M₁对小鼠肠道细菌多样性的影响[J]. 《中国畜牧兽医》, 2019, 46(2): 433-441.

ZHANG Muchen, ZHENG Nan, ZHAO Shengguo, ZHANG Yangdong, LI Songli, WANG Jiaqi. Effects of Aflatoxin B₁ and M₁ on Intestinal Bacterial Diversity in Mice[J]. , 2019, 46(2): 433-441.

参考文献

[1] PROBST C,NJAPAU H,COTTY P J.Outbreak of an acute aflatoxicosis in Kenya in 2004:Identification of the causal agent[J].Applied & Environmental Microbiology,2007,73(8):2762-2764.
[2] ALSHANNAQ A,YU J H.Occurrence,toxicity,and analysis of major mycotoxins in food[J].International Journal of Environmental Research & Public Health,2017,14(6):632-638.
[3] COTTY P J,JAIME-GARCIA R.Influences of climate on aflatoxin producing fungi and aflatoxin contamination[J].International Journal of Food Microbiology,2007,119(1):109-115.
[4] ALBENBERG L,ESIPOVA T V,JUDGE C P,et al.Correlation between intraluminal oxygen gradient and radial partitioning of intestinal microbiota in humans and mice[J].Gastroenterology,2014,147(5):1055-1063.
[5] LEE H J,RYU D.Worldwide occurrence of mycotoxins in cereals and cereal derived food products:Public health perspectives of their co-occurrence[J].Journal of Agricultural and Food Chemistry,2016,65(33):7034-7051.
[6] 张维蔚,何洁仪,李迎月,等.2009-2013年广州市市售粮油食品黄曲霉毒素B1调查[J].中国食品卫生杂志,2015,27(3):291-294. ZHANG W W,HE J Y,LI Y Y,et al.Investigation on aflatoxin B1 in cereals,oils and foodstuffs sold in Guangzhou from 2009 to 2013[J]. Chinese Journal of Food Hygiene.2015,27(3):291-294.(in Chinese)
[7] HUSSAIN I,ANWAR J,ASI M R,et al.Aflatoxin M1 contamination in milk from five dairy species in Pakistan[J].Food Control,2010,21(2):122-124.
[8] IHA M H,BARBOSA C B,OKADA I A,et al.Aflatoxin M1 in milk and distribution and stability of aflatoxin M1 during production and storage of yoghurt and cheese[J].Food Control,2013,29(1):1-6.
[9] 蔡丽云,黄泽彬,须子唯,等.处理垃圾渗滤液的SBR中微生物种群与污泥比阻[J].环境科学,2018,39(2):880-887. CAI L Y,HUANG Z B,XU Z W,et al.Microbial communities and sludge specific resistance in two SBRs treating leachate[J].Environmental Science,2018,39(2):880-887.(in Chinese)
[10] 覃初斌.干酪乳杆菌对斑马鱼抵御气单胞菌感染的分子机制研究[D].杭州:浙江大学,2017. QIN C B.Molecular mechanism of lactobacillus casei against aeromonas infection in zebrafish[D].Hangzhou:Zhejiang University,2017.(in Chinese)
[11] PRANDINI A,TANSINI G,SIGOLO S,et al.On the occurrence of aflatoxin M1 in milk and dairy products[J].Food and Chemical Toxicology,2009,47(5):984-991.
[12] OUWERKERK J P,VOS W M D,BELZER C.Glycobiome:Bacteria and mucus at the epithelial interface[J].Best Practice & Research Clinical Gastroenterology,2013,27(1):25-38.
[13] OSWALD I P,MARIN D E,BOUHET S,et al.Immunotoxicological risk of mycotoxins for domestic animals[J].Food Additives and Contaminants,2005,22(4):354-360.
[14] MATTES W B,PETTIT S D,SANSONE S A,et al.Database development in toxicogenomics:Issues and efforts[J].Environmental Health Perspectives,2004,112(4):495-505.
[15] MOTAWEE M M,BAUER J,MCMAHON D J.Survey of aflatoxin M1 in cow,goat,buffalo and camel milks in Ismailia-Egypt[J].Bulletin of Environmental Contamination and Toxicology,2009,83(5):766-769.
[16] MAGNUSSEN A,PARSI M A.Aflatoxins,hepatocellular carcinoma and public health[J].World Journal of Gastroenterology,2013,19(10):1508-1512.
[17] LEE H J,RYU D.Worldwide occurrence of mycotoxins in cereals and cereal derived food products:Public health perspectives of their co-occurrence[J].Journal of Agricultural and Food Chemistry,2016,65(33):7034-7051.
[18] FADROSH D W,MA B,GAJER P,et al.An improved dual-indexing approach for multiplexed 16S rRNA gene sequencing on the Illumina MiSeq platform[J].Microbiome,2014,2(1):6-11.
[19] TREMBLAY J,SINGH K,FERN A,et al.Primer and platform effects on 16S rRNA tag sequencing[J].Frontiers in Microbiology,2015,6:771-777.
[20] SMETS W,LEFF J W,BRADFORD M A,et al.A method for simultaneous measurement of soil bacterial abundances and community composition via 16S rRNA gene sequencing[J].Soil Biology and Biochemistry,2016,96:145-151.
[21] WALKER A W,MARTIN J C,SCOTT P,et al.16S rRNA gene-based profiling of the human infant gut microbiota is strongly influenced by sample processing and PCR primer choice[J].Microbiome,2015,3(1):26-31.
[22] PEREZ-LOPEZ A,BEHNSEN J,NUCCIO S P,et al.Mucosal immunity to pathogenic intestinal bacteria[J].Nature Reviews Immunology,2016,16(3):135-142.
[23] KAMADA N,NÚÑEZ G.Regulation of the immune system by the resident intestinal bacteria[J].Gastroenterology,2014,146(6):1477-1488.
[24] SARTOR R B,WU G D.Roles for intestinal bacteria,viruses,and fungi in pathogenesis of inflammatory bowel diseases and therapeutic approaches[J].Gastroenterology,2017,152(2):327-339.
[25] RIDLON J M,HARRIS S C,BHOWMIK S,et al.Consequences of bile salt biotransformations by intestinal bacteria[J].Gut Microbes,2016,7(1):22-39.
[26] WEBB C R,KOBOZIEV I,FURR K L,et al.Protective and pro-inflammatory roles of intestinal bacteria[J].Pathophysiology,2016,23(2):67-80.
[27] CUMMINGS J H,MACFARLANE G T.Role of intestinal bacteria in nutrient metabolism[J].Clinical nutrition,1997,16(1):3-11.
[28] LYNCH S V,PEDERSEN O.The human intestinal microbiome in health and disease[J].New England Journal of Medicine,2016,375(24):2369-2379.
[29] WANG J C,TANG L L,GLENN T C,et al.Aflatoxin B1 induced compositional changes in gut microbial communities of male F344 rats[J].Toxicological Sciences,2015,150(1):54-63.

黄曲霉毒素B₁与M₁对小鼠肠道细菌多样性的影响

Effects of Aflatoxin B₁ and M₁ on Intestinal Bacterial Diversity in Mice

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