肌肉生长抑制素在小鼠不同发育阶段心肌组织中的表达

doi:10.16431/j.cnki.1671-7236.2018.06.007

摘要/Abstract

摘要：

本试验旨在探究肌肉生长抑制素（myostatin，MSTN）基因在小鼠不同发育阶段心肌组织中的表达情况，分别选取幼年（7日龄）、性成熟（21~28日龄）、体成熟（42~56日龄）和老年（84日龄以上）4个发育阶段的健康小鼠（各3只）为研究对象，采用实时荧光定量PCR、HE染色、免疫组织化学等方法研究小鼠不同发育时期MSTN基因在心肌组织中的表达及不同时期心肌组织的发育。实时荧光定量PCR结果显示，MSTN基因在小鼠不同发育阶段心肌组织中均有表达，且其在小鼠老年时期的表达量最高，体成熟时期的表达量最低；幼年小鼠和性成熟小鼠心肌组织中MSTN基因表达水平显著低于老年小鼠（P<0.05），而体成熟小鼠则极显著性低于老年小鼠（P<0.01）。HE染色结果显示，小鼠心肌细胞核及肌纤维的生长在不同发育时期明显不同，其中幼年时期的细胞核最多，肌纤维排列相对较紧密，而在性成熟、体成熟、老年时期心肌的发育指标依次降低。免疫组织化学结果显示，MSTN基因主要在心肌细胞核中表达，其各个时期表达量与实时荧光定量PCR的结果相符合。

关键词: 肌肉生长抑制素; 心肌; 表达分布

Abstract:

The aim of this experiment was to investigate the expression of myostatin (MSTN) gene in mouse myocardium at different developmental stages.12 healthy mice at different developmental stages were chosen,including young (7 days old),sexual mature (21 to 28 days old), somatic mature (42 to 56 days old) and aged mice (84 days old or older),with three mice per developmental stage.The expression of MSTN gene in mice myocardium and myocardial development were detected by quantitative Real-time PCR,HE staining and immunohistochemistry.The results of quantitative Real-time PCR showed that MSTN gene could express in myocardium tissue of mice at all different developmental stages.And it was the highest in aged mice and the lowest in somatic mature mice.Compared with the aged mice,the expression of MSTN gene in juvenile mice and sexually mature mice was significantly decreased (P<0.05), however,it was extremely significantly decreased in somatic mature mice (P<0.01).The results of HE staining showed that the growth of myocardial nuclei and muscle fibers were differences in different ages.Among them,the nucleus was the most and the muscle fiber arrangement was relatively close in juvenile mice,but the indexes of myocardial development in sexually mature mice,somatic mature mice and aged mice were decreased in turn.Immunohistochemical results showed that MSTN was mainly expressed in the nucleus of myocardium,and the level of its expression in each stage was consistent with the results of quantitative Real-time PCR.

Key words: myostatin (MSTN); myocardium; expression distribution

中图分类号:

薛霖莉, 李艺雷, 候亚琴, 冀云燕, 任华伟, 耿建军, 王海东, 赫晓燕, 邢海云. 肌肉生长抑制素在小鼠不同发育阶段心肌组织中的表达[J]. 《中国畜牧兽医》, 2018, 45(6): 1479-1485.

XUE Linli, LI Yilei, HOU Yaqin, JI Yunyan, REN Huawei, GENG Jianjun, WANG Haidong, HE Xiaoyan, XING Haiyun. Expression of Myostatin in Myocardium of Mice at Different Developmental Stages[J]. , 2018, 45(6): 1479-1485.

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