芦花鸡IFITM3基因克隆及生物信息学分析

doi:10.16431/j.cnki.1671-7236.2018.02.006

《中国畜牧兽医》 ›› 2018, Vol. 45 ›› Issue (2): 330-337.doi: 10.16431/j.cnki.1671-7236.2018.02.006

芦花鸡IFITM3基因克隆及生物信息学分析

姜莉莉^1,2, 许腾², 周宛蓉², 樊兆斌²

1. 锦州医科大学畜牧兽医学院, 锦州 121001;
2. 菏泽学院药学院, 菏泽 274015

收稿日期:2017-05-18 出版日期:2018-02-20 发布日期:2018-02-10
通讯作者: 樊兆斌 E-mail:252892335@qq.com
作者简介:姜莉莉(1981-),女,山东烟台人,博士,研究方向:分子病毒学,E-mail:lyjllfxy1024@163.com
基金资助:
国家自然科学基金项目（31402170）；辽宁省自然科学基金项目（2014022056）；山东省高等学校科研计划项目（J17KA126）；山东省自然科学基金面上项目（ZR2017MC055）；国家大学生创新训练项目（201710455158）；菏泽学院博士基金（XY16BS09）

Cloning and Bioinformatics Analysis of Interferon-induced Transmembrane Protein 3(IFITM3) Gene in Luhua Chicken

JIANG Lili^1,2, XU Teng², ZHOU Wanrong², FAN Zhaobin²

1. Institute of Animal Husbandry Veterinary, Jinzhou Medical University, Jinzhou 121001, China;
2. College of Pharmacy, Heze University, Heze 274015, China

Received:2017-05-18 Online:2018-02-20 Published:2018-02-10

摘要/Abstract

摘要：

本研究旨在对芦花鸡的干扰素诱导的跨膜蛋白-3（interferon-induced transmembrane protein 3，IFITM3）基因进行克隆及生物信息学分析。根据GenBank中鸡IFITM3基因序列（登录号：NM_001350061.1）设计特异性引物，利用RT-PCR技术对芦花鸡IFITM3基因进行克隆、测序和生物信息学分析。结果表明，芦花鸡IFITM3基因片段大小为414 bp，与GenBank中发表的鸡IFITM3基因序列（登录号：NM_001350061.1）同源性为99.9%；编码137个氨基酸。IFITM3基因理论分子质量为14.95 ku，理论等电点（pI）为6.89，不稳定系数为33.98，脂肪系数为103.14，平均疏水指数为0.300，存在3个明显的疏水区，无信号肽，存在2个跨膜区，分别位于第46-68、101-123位氨基酸处；二级结构预测显示，IFITN3蛋白主要以无规则卷曲为主，存在多个α-螺旋和β-折叠区域。本研究成功克隆了芦花鸡IFITM3基因，为进一步研究IFITM3蛋白功能及阐明其抗病毒分子机制奠定了理论基础。

关键词: 干扰素诱导的跨膜蛋白-3(IFITM3)基因; 克隆; 序列分析; 生物信息学分析

Abstract:

This study was aimed to clone the interferon-induced transmembrane protein 3(IFITM3) gene of Luhua chicken and analyze its genetic structure with bioinformatics. One pair of special primers were designed according to predicted sequence of chicken IFITM3 gene in GenBank (accession No.:NM_001350061.1). The IFITM3 gene of Luhua chicken was amplified by RT-PCR, the physicochemical property and secondary structure of IFITM3 gene were systemically analyzed by bioinformatics. The results showed that the IFITM3 gene fragment included a 414 bp whole length CDS (coding 137 bp amino acids), and the gene sequence homology was above 99.9% comparison with the sequence published in GenBank (accession No.:NM_001350061.1). The molecular weight, isoelectric point, instability index and fat coefficient of IFITM3 were 14.95 ku,6.89,33.98 and 103.14, respectively. The prediction result of IFITM3 gene structure showed that IFITM3 had no signal peptide but had three obvious hydrophobic domains, and the average hydrophobic index was 0.300. The two transmembrane domains might locate in the 46 to 68 and 101 to 123 amino acids across the online TMHMM 2.0 software. The prediction of secondary structure revealed that some α-helixes and β-sheets exist in IFITM3 protein while random coil was major pattern. The results suggested that the whole CDS sequence of Luhua chicken IFITM3 gene was successfully cloned, which provided foundation for further studying on the function and antiviral molecular mechanisms of Luhua chicken IFITM3 protein.

Key words: IFITM3 gene; clone; sequence analysis; bioinformatics analysis

中图分类号:

姜莉莉, 许腾, 周宛蓉, 樊兆斌. 芦花鸡IFITM3基因克隆及生物信息学分析[J]. 《中国畜牧兽医》, 2018, 45(2): 330-337.

JIANG Lili, XU Teng, ZHOU Wanrong, FAN Zhaobin. Cloning and Bioinformatics Analysis of Interferon-induced Transmembrane Protein 3(IFITM3) Gene in Luhua Chicken[J]. , 2018, 45(2): 330-337.

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芦花鸡IFITM3基因克隆及生物信息学分析

Cloning and Bioinformatics Analysis of Interferon-induced Transmembrane Protein 3(IFITM3) Gene in Luhua Chicken

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