不同生物型BVDV感染宿主细胞后差异基因分析

doi:10.16431/j.cnki.1671-7236.2017.11.001

《中国畜牧兽医》 ›› 2017, Vol. 44 ›› Issue (11): 3113-3120.doi: 10.16431/j.cnki.1671-7236.2017.11.001

• 生物技术 • 下一篇

不同生物型BVDV感染宿主细胞后差异基因分析

程凯慧, 朱彤, 侯佩莉, 王洪梅, 苗自利, 张亮, 解晓莉, 杨宏军, 何洪彬

山东省农业科学院奶牛研究中心, 济南 250131

收稿日期:2017-04-07 出版日期:2017-11-20 发布日期:2017-11-21
通讯作者: 杨宏军, 何洪彬 E-mail:longfei1997@sina.com;hongbinh@hotmail.com
作者简介:程凯慧(1982-),女,山东平度人,博士,助理研究员,研究方向:病毒分子生物学,E-mail:chengkaihui@126.com
基金资助:
十三五国家重点研发计划（2016YFD0500904）；山东省优秀中青年科学家科研奖励基金（BS2014SW008）；现代农业（奶牛）产业技术体系科学家岗位（CARS-36）；山东省农科院青年基金（2014QNM17）；山东省自然基金（ZR2016CP09）；山东省农业科学院农业科技创新工程（CXGC2016B14、CXGC2016A10）

Differential Gene Analysis of Host Cell Infected by Different Biotypes of Bovine Viral Diarrhea Virus

CHENG Kai-hui, ZHU Tong, HOU Pei-li, WAMG Hong-mei, MIAO Zi-li, ZHANG Liang, XIE Xiao-li, YANG Hong-jun, HE Hong-bin

Dairy Cattle Research Center, Shandong Academy of Agricultural Sciences, Jinan 250131, China

Received:2017-04-07 Online:2017-11-20 Published:2017-11-21

摘要/Abstract

摘要：

为深入研究牛场中普遍存在的持续性感染，探讨不同生物型牛病毒性腹泻病毒（bovine viral diarrhea virus，BVDV）转化的分子机制，本试验将致细胞病变型BVDV （CP型BVDV）和非致细胞病变型BVDV （NCP型BVDV）感染MDBK细胞，观察细胞变化，感染后12~72 h期间每间隔12 h收集1次细胞，同时对24、48 h收集的细胞进行转录组学分析。结果显示，不同生物型BVDV感染宿主细胞24 h后，与感染NCP型BVDV的细胞相比，感染CP型BVDV的MDBK细胞中上调差异表达的基因2 849个，下调差异表达的基因3 347个，48 h后，上调差异表达的基因2 933个，下调差异表达的基因2 831个。对差异基因的GO功能分析结果表明，差异基因参与的分子功能主要有催化活性、结合活性、酶调节活性、分子转导活性和蛋白结合转录因子活性等；Pathway显著性富集分析结果显示，差异表达基因主要参与细胞自噬、细胞凋亡、免疫调节因子等相关的信号通路。本试验结果可为进一步揭示病毒致病的分子机制、控制BVDV和研制其候选疫苗奠定基础。

关键词: 致细胞病变型BVDV; 非致细胞病变型BVDV; 差异基因; GO功能分析; Pathway显著性分析

Abstract:

In order to further study the problem of persistent infection and investigate the molecular mechanism of transformation between different biotypes of bovine viral diarrhea virus(BVDV),cytopathic BVDV (CP BVDV) and non cytopathic BVDV (NCP BVDV) were used to infect MDBK host cell and the cells were observed and collected per 12 h from 12 to 72 h,and at the same time the transcriptome analysis were used to analyse the 24 and 48 h MDBK cells.The results showed that at 24 h,compared to MDBK cell infected by NCP BVDV,there were 2 849 up-regulated and 3 347 down-regulated differential genes,while 2 933 up-regulated and 2 831 down-regulated differential genes at 48 h in MDBK cell infected by CP BVDV.The results of GO function classification statistics showed that the molecular function of differential gene mainly involved in catalytic activity,binding activity, enzyme regulator activity,molecular transduction activity and protein binding transcription factor activity. Pathway saliency map showed that the genes involved in autophagy, apoptosis, signal transduction related expression of immunoregulatory factor were preliminary screened. The found of differential gene laid the foundation to further reveal the molecular mechanism of viral pathogenesis,also provided molecular targets for the development of new anti BVDV preparations.

Key words: cytopathogenic BVDV (CP BVDV); non cytopathic BVDV (NCP BVDV); differential gene; GO function analysis; Pathway analysis

中图分类号:

S855.3

程凯慧, 朱彤, 侯佩莉, 王洪梅, 苗自利, 张亮, 解晓莉, 杨宏军, 何洪彬. 不同生物型BVDV感染宿主细胞后差异基因分析[J]. 《中国畜牧兽医》, 2017, 44(11): 3113-3120.

CHENG Kai-hui, ZHU Tong, HOU Pei-li, WAMG Hong-mei, MIAO Zi-li, ZHANG Liang, XIE Xiao-li, YANG Hong-jun, HE Hong-bin. Differential Gene Analysis of Host Cell Infected by Different Biotypes of Bovine Viral Diarrhea Virus[J]. , 2017, 44(11): 3113-3120.

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不同生物型BVDV感染宿主细胞后差异基因分析

Differential Gene Analysis of Host Cell Infected by Different Biotypes of Bovine Viral Diarrhea Virus

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